Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus

Halobacillus halophilus, a moderately halophilic bacterium isolated from salt marshes, produces various compatible solutes to cope with osmotic stress. Glutamate and glutamine are dominant compatible solutes at mild salinities. Glutamine synthetase activity in cell suspensions of Halobacillus haloph...

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Main Authors:	Shiyan, Anna, Thompson, Melanie, Köcher, Saskia, Tausendschön, Michaela, Santos, Helena, Hänelt, Inga, Müller, Volker
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995056/

id	pubmed-3995056
recordtype	oai_dc
spelling	pubmed-39950562014-04-29 Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus Shiyan, Anna Thompson, Melanie Köcher, Saskia Tausendschön, Michaela Santos, Helena Hänelt, Inga Müller, Volker Microbiology Halobacillus halophilus, a moderately halophilic bacterium isolated from salt marshes, produces various compatible solutes to cope with osmotic stress. Glutamate and glutamine are dominant compatible solutes at mild salinities. Glutamine synthetase activity in cell suspensions of Halobacillus halophilus wild type was shown to be salt dependent and chloride modulated. A possible candidate to catalyze glutamine synthesis is glutamine synthetase A2, whose transcription is stimulated by chloride. To address the role of GlnA2 in the biosynthesis of the osmolytes glutamate and glutamine, a deletion mutant (ΔglnA2) was generated and characterized in detail. We compared the pool of compatible solutes and performed transcriptional analyses of the principal genes controlling the solute production in the wild type strain and the deletion mutant. These measurements did not confirm the hypothesized role of GlnA2 in the osmolyte production. Most likely the presence of another, yet to be identified enzyme has the main contribution in the measured activity in crude extracts and probably determines the total chloride-modulated profile. The role of GlnA2 remains to be elucidated. Frontiers Media S.A. 2014-04-14 /pmc/articles/PMC3995056/ /pubmed/24782854 http://dx.doi.org/10.3389/fmicb.2014.00168 Text en Copyright © 2014 Shiyan, Thompson, Köcher, Tausendschön, Santos, Hänelt and Müller. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Shiyan, Anna Thompson, Melanie Köcher, Saskia Tausendschön, Michaela Santos, Helena Hänelt, Inga Müller, Volker
spellingShingle	Shiyan, Anna Thompson, Melanie Köcher, Saskia Tausendschön, Michaela Santos, Helena Hänelt, Inga Müller, Volker Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
author_facet	Shiyan, Anna Thompson, Melanie Köcher, Saskia Tausendschön, Michaela Santos, Helena Hänelt, Inga Müller, Volker
author_sort	Shiyan, Anna
title	Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
title_short	Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
title_full	Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
title_fullStr	Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
title_full_unstemmed	Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
title_sort	glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in halobacillus halophilus
description	Halobacillus halophilus, a moderately halophilic bacterium isolated from salt marshes, produces various compatible solutes to cope with osmotic stress. Glutamate and glutamine are dominant compatible solutes at mild salinities. Glutamine synthetase activity in cell suspensions of Halobacillus halophilus wild type was shown to be salt dependent and chloride modulated. A possible candidate to catalyze glutamine synthesis is glutamine synthetase A2, whose transcription is stimulated by chloride. To address the role of GlnA2 in the biosynthesis of the osmolytes glutamate and glutamine, a deletion mutant (ΔglnA2) was generated and characterized in detail. We compared the pool of compatible solutes and performed transcriptional analyses of the principal genes controlling the solute production in the wild type strain and the deletion mutant. These measurements did not confirm the hypothesized role of GlnA2 in the osmolyte production. Most likely the presence of another, yet to be identified enzyme has the main contribution in the measured activity in crude extracts and probably determines the total chloride-modulated profile. The role of GlnA2 remains to be elucidated.
publisher	Frontiers Media S.A.
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995056/
_version_	1612080685358514176

Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus

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