Allergic sensitization: screening methods

Experimental in silico, in vitro, and rodent models for screening and predicting protein sensitizing potential are discussed, including whether there is evidence of new sensitizations and allergies since the introduction of genetically modified crops in 1996, the importance of linear versus conforma...

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Main Authors:	Ladics, Gregory S, Fry, Jeremy, Goodman, Richard, Herouet-Guicheney, Corinne, Hoffmann-Sommergruber, Karin, Madsen, Charlotte B, Penninks, André, Pomés, Anna, Roggen, Erwin L, Smit, Joost, Wal, Jean-Michel
Format:	Online
Language:	English
Published:	BioMed Central 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990213/

id	pubmed-3990213
recordtype	oai_dc
spelling	pubmed-39902132014-04-18 Allergic sensitization: screening methods Ladics, Gregory S Fry, Jeremy Goodman, Richard Herouet-Guicheney, Corinne Hoffmann-Sommergruber, Karin Madsen, Charlotte B Penninks, André Pomés, Anna Roggen, Erwin L Smit, Joost Wal, Jean-Michel Review Experimental in silico, in vitro, and rodent models for screening and predicting protein sensitizing potential are discussed, including whether there is evidence of new sensitizations and allergies since the introduction of genetically modified crops in 1996, the importance of linear versus conformational epitopes, and protein families that become allergens. Some common challenges for predicting protein sensitization are addressed: (a) exposure routes; (b) frequency and dose of exposure; (c) dose-response relationships; (d) role of digestion, food processing, and the food matrix; (e) role of infection; (f) role of the gut microbiota; (g) influence of the structure and physicochemical properties of the protein; and (h) the genetic background and physiology of consumers. The consensus view is that sensitization screening models are not yet validated to definitively predict the de novo sensitizing potential of a novel protein. However, they would be extremely useful in the discovery and research phases of understanding the mechanisms of food allergy development, and may prove fruitful to provide information regarding potential allergenicity risk assessment of future products on a case by case basis. These data and findings were presented at a 2012 international symposium in Prague organized by the Protein Allergenicity Technical Committee of the International Life Sciences Institute’s Health and Environmental Sciences Institute. BioMed Central 2014-04-15 /pmc/articles/PMC3990213/ /pubmed/24739743 http://dx.doi.org/10.1186/2045-7022-4-13 Text en Copyright © 2014 Ladics et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ladics, Gregory S Fry, Jeremy Goodman, Richard Herouet-Guicheney, Corinne Hoffmann-Sommergruber, Karin Madsen, Charlotte B Penninks, André Pomés, Anna Roggen, Erwin L Smit, Joost Wal, Jean-Michel
spellingShingle	Ladics, Gregory S Fry, Jeremy Goodman, Richard Herouet-Guicheney, Corinne Hoffmann-Sommergruber, Karin Madsen, Charlotte B Penninks, André Pomés, Anna Roggen, Erwin L Smit, Joost Wal, Jean-Michel Allergic sensitization: screening methods
author_facet	Ladics, Gregory S Fry, Jeremy Goodman, Richard Herouet-Guicheney, Corinne Hoffmann-Sommergruber, Karin Madsen, Charlotte B Penninks, André Pomés, Anna Roggen, Erwin L Smit, Joost Wal, Jean-Michel
author_sort	Ladics, Gregory S
title	Allergic sensitization: screening methods
title_short	Allergic sensitization: screening methods
title_full	Allergic sensitization: screening methods
title_fullStr	Allergic sensitization: screening methods
title_full_unstemmed	Allergic sensitization: screening methods
title_sort	allergic sensitization: screening methods
description	Experimental in silico, in vitro, and rodent models for screening and predicting protein sensitizing potential are discussed, including whether there is evidence of new sensitizations and allergies since the introduction of genetically modified crops in 1996, the importance of linear versus conformational epitopes, and protein families that become allergens. Some common challenges for predicting protein sensitization are addressed: (a) exposure routes; (b) frequency and dose of exposure; (c) dose-response relationships; (d) role of digestion, food processing, and the food matrix; (e) role of infection; (f) role of the gut microbiota; (g) influence of the structure and physicochemical properties of the protein; and (h) the genetic background and physiology of consumers. The consensus view is that sensitization screening models are not yet validated to definitively predict the de novo sensitizing potential of a novel protein. However, they would be extremely useful in the discovery and research phases of understanding the mechanisms of food allergy development, and may prove fruitful to provide information regarding potential allergenicity risk assessment of future products on a case by case basis. These data and findings were presented at a 2012 international symposium in Prague organized by the Protein Allergenicity Technical Committee of the International Life Sciences Institute’s Health and Environmental Sciences Institute.
publisher	BioMed Central
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990213/
_version_	1612079274585489408

Allergic sensitization: screening methods

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