Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris☆
•A novel 2-step purification for recombinant HRP from P. pastrois was developed.•Both purification steps are negative chromatography steps.•The amount of N-glycosylation sites and the success in purification correlate.•HRP isoenzymes differ significantly in substrate specificity and catalytic activi...
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Bibliographic Details
| Main Authors: |
Krainer, Florian W.,
Pletzenauer, Robert,
Rossetti, Laura,
Herwig, Christoph,
Glieder, Anton,
Spadiut, Oliver |
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| Format: | Online
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| Language: | English |
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| Published: |
Academic Press
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989067/
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