Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris☆
•A novel 2-step purification for recombinant HRP from P. pastrois was developed.•Both purification steps are negative chromatography steps.•The amount of N-glycosylation sites and the success in purification correlate.•HRP isoenzymes differ significantly in substrate specificity and catalytic activi...
| Main Authors: | , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Academic Press
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989067/ |