Replication Origin Selection Regulates the Distribution of Meiotic Recombination
The program of DNA replication, defined by the temporal and spatial pattern of origin activation, is altered during development and in cancers. However, whether changes in origin usage play a role in regulating specific biological processes remains unknown. We investigated the consequences of modify...
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| Format: | Online |
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Cell Press
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988929/ |
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pubmed-39889292014-04-17 Replication Origin Selection Regulates the Distribution of Meiotic Recombination Wu, Pei-Yun Jenny Nurse, Paul Short Article The program of DNA replication, defined by the temporal and spatial pattern of origin activation, is altered during development and in cancers. However, whether changes in origin usage play a role in regulating specific biological processes remains unknown. We investigated the consequences of modifying origin selection on meiosis in fission yeast. Genome-wide changes in the replication program of premeiotic S phase do not affect meiotic progression, indicating that meiosis neither activates nor requires a particular origin pattern. In contrast, local changes in origin efficiencies between different replication programs lead to changes in Rad51 recombination factor binding and recombination frequencies in these domains. We observed similar results for Rad51 when changes in efficiencies were generated by directly targeting expression of the Cdc45 replication factor. We conclude that origin selection is a key determinant for organizing meiotic recombination, providing evidence that genome-wide modifications in replication program can modulate cellular physiology. Cell Press 2014-02-20 /pmc/articles/PMC3988929/ /pubmed/24560273 http://dx.doi.org/10.1016/j.molcel.2014.01.022 Text en © 2014 Elsevier Inc. All rights reserved. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wu, Pei-Yun Jenny Nurse, Paul |
| spellingShingle |
Wu, Pei-Yun Jenny Nurse, Paul Replication Origin Selection Regulates the Distribution of Meiotic Recombination |
| author_facet |
Wu, Pei-Yun Jenny Nurse, Paul |
| author_sort |
Wu, Pei-Yun Jenny |
| title |
Replication Origin Selection Regulates the Distribution of Meiotic Recombination |
| title_short |
Replication Origin Selection Regulates the Distribution of Meiotic Recombination |
| title_full |
Replication Origin Selection Regulates the Distribution of Meiotic Recombination |
| title_fullStr |
Replication Origin Selection Regulates the Distribution of Meiotic Recombination |
| title_full_unstemmed |
Replication Origin Selection Regulates the Distribution of Meiotic Recombination |
| title_sort |
replication origin selection regulates the distribution of meiotic recombination |
| description |
The program of DNA replication, defined by the temporal and spatial pattern of origin activation, is altered during development and in cancers. However, whether changes in origin usage play a role in regulating specific biological processes remains unknown. We investigated the consequences of modifying origin selection on meiosis in fission yeast. Genome-wide changes in the replication program of premeiotic S phase do not affect meiotic progression, indicating that meiosis neither activates nor requires a particular origin pattern. In contrast, local changes in origin efficiencies between different replication programs lead to changes in Rad51 recombination factor binding and recombination frequencies in these domains. We observed similar results for Rad51 when changes in efficiencies were generated by directly targeting expression of the Cdc45 replication factor. We conclude that origin selection is a key determinant for organizing meiotic recombination, providing evidence that genome-wide modifications in replication program can modulate cellular physiology. |
| publisher |
Cell Press |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988929/ |
| _version_ |
1612078882856370176 |