A ribozyme that triphosphorylates RNA 5′-hydroxyl groups
The RNA world hypothesis describes a stage in the early evolution of life in which RNA served as genome and as the only genome-encoded catalyst. To test whether RNA world organisms could have used cyclic trimetaphosphate as an energy source, we developed an in vitro selection strategy for isolating...
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Oxford University Press
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985629/ |
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pubmed-39856292014-04-18 A ribozyme that triphosphorylates RNA 5′-hydroxyl groups Moretti, Janina E. Müller, Ulrich F. Synthetic Biology and Chemistry The RNA world hypothesis describes a stage in the early evolution of life in which RNA served as genome and as the only genome-encoded catalyst. To test whether RNA world organisms could have used cyclic trimetaphosphate as an energy source, we developed an in vitro selection strategy for isolating ribozymes that catalyze the triphosphorylation of RNA 5′-hydroxyl groups with trimetaphosphate. Several active sequences were isolated, and one ribozyme was analyzed in more detail. The ribozyme was truncated to 96 nt, while retaining full activity. It was converted to a trans-format and reacted with rates of 0.16 min−1 under optimal conditions. The secondary structure appears to contain a four-helical junction motif. This study showed that ribozymes can use trimetaphosphate to triphosphorylate RNA 5′-hydroxyl groups and suggested that RNA world organisms could have used trimetaphosphate as their energy source. Oxford University Press 2014-04 2014-01-21 /pmc/articles/PMC3985629/ /pubmed/24452796 http://dx.doi.org/10.1093/nar/gkt1405 Text en © The Author(s) 2014. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Moretti, Janina E. Müller, Ulrich F. |
| spellingShingle |
Moretti, Janina E. Müller, Ulrich F. A ribozyme that triphosphorylates RNA 5′-hydroxyl groups |
| author_facet |
Moretti, Janina E. Müller, Ulrich F. |
| author_sort |
Moretti, Janina E. |
| title |
A ribozyme that triphosphorylates RNA 5′-hydroxyl groups |
| title_short |
A ribozyme that triphosphorylates RNA 5′-hydroxyl groups |
| title_full |
A ribozyme that triphosphorylates RNA 5′-hydroxyl groups |
| title_fullStr |
A ribozyme that triphosphorylates RNA 5′-hydroxyl groups |
| title_full_unstemmed |
A ribozyme that triphosphorylates RNA 5′-hydroxyl groups |
| title_sort |
ribozyme that triphosphorylates rna 5′-hydroxyl groups |
| description |
The RNA world hypothesis describes a stage in the early evolution of life in which RNA served as genome and as the only genome-encoded catalyst. To test whether RNA world organisms could have used cyclic trimetaphosphate as an energy source, we developed an in vitro selection strategy for isolating ribozymes that catalyze the triphosphorylation of RNA 5′-hydroxyl groups with trimetaphosphate. Several active sequences were isolated, and one ribozyme was analyzed in more detail. The ribozyme was truncated to 96 nt, while retaining full activity. It was converted to a trans-format and reacted with rates of 0.16 min−1 under optimal conditions. The secondary structure appears to contain a four-helical junction motif. This study showed that ribozymes can use trimetaphosphate to triphosphorylate RNA 5′-hydroxyl groups and suggested that RNA world organisms could have used trimetaphosphate as their energy source. |
| publisher |
Oxford University Press |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985629/ |
| _version_ |
1612077698086076416 |