Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice

Circadian clocks are the endogenous oscillators that harmonize a variety of physiological processes within the body. Although many urinary functions exhibit clear daily or circadian variation in diurnal humans and nocturnal rodents, the precise mechanisms of these variations are as yet unclear. In t...

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Main Authors:	Noh, Jong-Yun, Han, Dong-Hee, Kim, Mi-Hee, Ko, Il-Gyu, Kim, Sung-Eun, Park, Noheon, Kyoung Choe, Han, Kim, Khae-Hawn, Kim, Kyungjin, Kim, Chang-Ju, Cho, Sehyung
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972783/

id	pubmed-3972783
recordtype	oai_dc
spelling	pubmed-39727832014-04-02 Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice Noh, Jong-Yun Han, Dong-Hee Kim, Mi-Hee Ko, Il-Gyu Kim, Sung-Eun Park, Noheon Kyoung Choe, Han Kim, Khae-Hawn Kim, Kyungjin Kim, Chang-Ju Cho, Sehyung Original Article Circadian clocks are the endogenous oscillators that harmonize a variety of physiological processes within the body. Although many urinary functions exhibit clear daily or circadian variation in diurnal humans and nocturnal rodents, the precise mechanisms of these variations are as yet unclear. In the present study, we demonstrate that Per2 promoter activity clearly oscillates in neonate and adult bladders cultured ex vivo from Per2::Luc knock-in mice. In subsequent experiments, we show that multiple local oscillators are operating in all the bladder tissues (detrusor, sphincter and urothelim) and the lumbar spinal cord (L4–5) but not in the pontine micturition center or the ventrolateral periaqueductal gray of the brain. Accordingly, the water intake and urine volume exhibited daily and circadian variations in young adult wild-type mice but not in Per1−/−Per2−/− mice, suggesting a functional clock-dependent nature of the micturition rhythm. Particularly in PDK mice, the water intake and urinary excretion displayed an arrhythmic pattern under constant darkness, and the amount of water consumed and excreted significantly increased compared with those of WT mice. These results suggest that local circadian clocks reside in three types of bladder tissue and the lumbar spinal cord and may have important roles in the circadian control of micturition function. Nature Publishing Group 2014-03 2014-03-07 /pmc/articles/PMC3972783/ /pubmed/24603368 http://dx.doi.org/10.1038/emm.2013.153 Text en Copyright © 2014 KSBMB. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Noh, Jong-Yun Han, Dong-Hee Kim, Mi-Hee Ko, Il-Gyu Kim, Sung-Eun Park, Noheon Kyoung Choe, Han Kim, Khae-Hawn Kim, Kyungjin Kim, Chang-Ju Cho, Sehyung
spellingShingle	Noh, Jong-Yun Han, Dong-Hee Kim, Mi-Hee Ko, Il-Gyu Kim, Sung-Eun Park, Noheon Kyoung Choe, Han Kim, Khae-Hawn Kim, Kyungjin Kim, Chang-Ju Cho, Sehyung Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
author_facet	Noh, Jong-Yun Han, Dong-Hee Kim, Mi-Hee Ko, Il-Gyu Kim, Sung-Eun Park, Noheon Kyoung Choe, Han Kim, Khae-Hawn Kim, Kyungjin Kim, Chang-Ju Cho, Sehyung
author_sort	Noh, Jong-Yun
title	Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
title_short	Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
title_full	Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
title_fullStr	Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
title_full_unstemmed	Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
title_sort	presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
description	Circadian clocks are the endogenous oscillators that harmonize a variety of physiological processes within the body. Although many urinary functions exhibit clear daily or circadian variation in diurnal humans and nocturnal rodents, the precise mechanisms of these variations are as yet unclear. In the present study, we demonstrate that Per2 promoter activity clearly oscillates in neonate and adult bladders cultured ex vivo from Per2::Luc knock-in mice. In subsequent experiments, we show that multiple local oscillators are operating in all the bladder tissues (detrusor, sphincter and urothelim) and the lumbar spinal cord (L4–5) but not in the pontine micturition center or the ventrolateral periaqueductal gray of the brain. Accordingly, the water intake and urine volume exhibited daily and circadian variations in young adult wild-type mice but not in Per1−/−Per2−/− mice, suggesting a functional clock-dependent nature of the micturition rhythm. Particularly in PDK mice, the water intake and urinary excretion displayed an arrhythmic pattern under constant darkness, and the amount of water consumed and excreted significantly increased compared with those of WT mice. These results suggest that local circadian clocks reside in three types of bladder tissue and the lumbar spinal cord and may have important roles in the circadian control of micturition function.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972783/
_version_	1612073183951716352

Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice

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