Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola
Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was m...
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Instituto Oswaldo Cruz, Ministério da Saúde
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970589/ |
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pubmed-39705892014-05-21 Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola Shimabukuro, Fabio Hiroto da Costa, Veruska Maia da Silva, Rodrigo Costa Langoni, Hélio da Silva, Aristeu Vieira de Carvalho, Lídia Raquel Domingues, Paulo Francisco Technical Notes Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs. Instituto Oswaldo Cruz, Ministério da Saúde 2013-08 /pmc/articles/PMC3970589/ /pubmed/23903987 http://dx.doi.org/10.1590/0074-0276108052013022 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Shimabukuro, Fabio Hiroto da Costa, Veruska Maia da Silva, Rodrigo Costa Langoni, Hélio da Silva, Aristeu Vieira de Carvalho, Lídia Raquel Domingues, Paulo Francisco |
| spellingShingle |
Shimabukuro, Fabio Hiroto da Costa, Veruska Maia da Silva, Rodrigo Costa Langoni, Hélio da Silva, Aristeu Vieira de Carvalho, Lídia Raquel Domingues, Paulo Francisco Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola |
| author_facet |
Shimabukuro, Fabio Hiroto da Costa, Veruska Maia da Silva, Rodrigo Costa Langoni, Hélio da Silva, Aristeu Vieira de Carvalho, Lídia Raquel Domingues, Paulo Francisco |
| author_sort |
Shimabukuro, Fabio Hiroto |
| title |
Prozone effects in microscopic agglutination tests for
leptospirosis in the sera of mice infected with the pathogenic
Leptospira interrogans serovar Canicola |
| title_short |
Prozone effects in microscopic agglutination tests for
leptospirosis in the sera of mice infected with the pathogenic
Leptospira interrogans serovar Canicola |
| title_full |
Prozone effects in microscopic agglutination tests for
leptospirosis in the sera of mice infected with the pathogenic
Leptospira interrogans serovar Canicola |
| title_fullStr |
Prozone effects in microscopic agglutination tests for
leptospirosis in the sera of mice infected with the pathogenic
Leptospira interrogans serovar Canicola |
| title_full_unstemmed |
Prozone effects in microscopic agglutination tests for
leptospirosis in the sera of mice infected with the pathogenic
Leptospira interrogans serovar Canicola |
| title_sort |
prozone effects in microscopic agglutination tests for
leptospirosis in the sera of mice infected with the pathogenic
leptospira interrogans serovar canicola |
| description |
Mice experimentally infected with a pathogenic strain of
Leptospira interrogans serovar Canicola produced false
negative results (prozone effect) in a microscopic agglutination test (MAT).
This prozone effect occurred in several serum samples collected at different
post-infection times, but it was more prominent in samples collected from
seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This
phenomenon was correlated with increased antibody titres in the early
post-infection phase. While prozone effects are often observed in serological
agglutination assays for the diagnosis of animal brucellosis and human syphilis,
they are not widely reported in leptospirosis MATs. |
| publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970589/ |
| _version_ |
1612072651865456640 |