In Vitro Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles
Cryopreservation of growing oocytes enriches the choice of timing and location of artificial embryo production. However, completion of oocyte growth after warming is crucial when using such cryopreserved oocytes. Our research objective was to develop a sequential system that incorporates cryopreser...
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| Format: | Online |
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The Society for Reproduction and Development
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963295/ |
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pubmed-39632952014-03-24 In Vitro Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles HIRAO, Yuji SOMFAI, Tamás NARUSE, Kenji Technology Report Cryopreservation of growing oocytes enriches the choice of timing and location of artificial embryo production. However, completion of oocyte growth after warming is crucial when using such cryopreserved oocytes. Our research objective was to develop a sequential system that incorporates cryopreservation of growing bovine oocytes and their subsequent in vitro growth. Oocyte-granulosa cell complexes with a mean oocyte diameter of approximately 100 µm were vitrified-warmed and then cultured for 14 days. The percentage of surviving oocytes following cryopreservation and 14-day culture was approximately 80%. More than half of the surviving oocytes were capable of maturing to metaphase II after in vitro maturation; the rate was comparable to that of control oocytes grown in vitro without cryopreservation. Taken together, the combined protocols for vitrification-warming of growing oocytes and subsequent in vitro growth can produce oocytes capable of undergoing meiotic maturation. The Society for Reproduction and Development 2013-10-11 2014-02 /pmc/articles/PMC3963295/ /pubmed/24126072 http://dx.doi.org/10.1262/jrd.2013-089 Text en ©2014 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
HIRAO, Yuji SOMFAI, Tamás NARUSE, Kenji |
| spellingShingle |
HIRAO, Yuji SOMFAI, Tamás NARUSE, Kenji In Vitro Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles |
| author_facet |
HIRAO, Yuji SOMFAI, Tamás NARUSE, Kenji |
| author_sort |
HIRAO, Yuji |
| title |
In Vitro Growth and Maturation of
Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles |
| title_short |
In Vitro Growth and Maturation of
Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles |
| title_full |
In Vitro Growth and Maturation of
Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles |
| title_fullStr |
In Vitro Growth and Maturation of
Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles |
| title_full_unstemmed |
In Vitro Growth and Maturation of
Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles |
| title_sort |
in vitro growth and maturation of
vitrified-warmed bovine oocytes collected from early antral follicles |
| description |
Cryopreservation of growing oocytes enriches the choice of timing and location of
artificial embryo production. However, completion of oocyte growth after warming is
crucial when using such cryopreserved oocytes. Our research objective was to develop a
sequential system that incorporates cryopreservation of growing bovine oocytes and their
subsequent in vitro growth. Oocyte-granulosa cell complexes with a mean
oocyte diameter of approximately 100 µm were vitrified-warmed and then cultured for 14
days. The percentage of surviving oocytes following cryopreservation and 14-day culture
was approximately 80%. More than half of the surviving oocytes were capable of maturing to
metaphase II after in vitro maturation; the rate was comparable to that
of control oocytes grown in vitro without cryopreservation. Taken
together, the combined protocols for vitrification-warming of growing oocytes and
subsequent in vitro growth can produce oocytes capable of undergoing
meiotic maturation. |
| publisher |
The Society for Reproduction and Development |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963295/ |
| _version_ |
1612070489282314240 |