In Vitro Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles
Cryopreservation of growing oocytes enriches the choice of timing and location of artificial embryo production. However, completion of oocyte growth after warming is crucial when using such cryopreserved oocytes. Our research objective was to develop a sequential system that incorporates cryopreser...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
The Society for Reproduction and Development
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963295/ |
| Summary: | Cryopreservation of growing oocytes enriches the choice of timing and location of
artificial embryo production. However, completion of oocyte growth after warming is
crucial when using such cryopreserved oocytes. Our research objective was to develop a
sequential system that incorporates cryopreservation of growing bovine oocytes and their
subsequent in vitro growth. Oocyte-granulosa cell complexes with a mean
oocyte diameter of approximately 100 µm were vitrified-warmed and then cultured for 14
days. The percentage of surviving oocytes following cryopreservation and 14-day culture
was approximately 80%. More than half of the surviving oocytes were capable of maturing to
metaphase II after in vitro maturation; the rate was comparable to that
of control oocytes grown in vitro without cryopreservation. Taken
together, the combined protocols for vitrification-warming of growing oocytes and
subsequent in vitro growth can produce oocytes capable of undergoing
meiotic maturation. |
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