The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC

The selection of vascular grafts for coronary artery bypass surgery is crucial for a positive outcome. This study aimed to establish a novel line of vascular endothelial cells with a potent anticoagulant effect. A lentiviral vector was used to stably transfect human umbilical vein endothelial cells...

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Main Authors:	Wang, Jian-Hua, Yuan, Lin-Jing, Zhong, Zhi-Min, Wen, Zhe-Sheng, Deng, Jian-Ming, Liang, Rong-Xin, Zheng, Min
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958884/

id	pubmed-3958884
recordtype	oai_dc
spelling	pubmed-39588842014-03-20 The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC Wang, Jian-Hua Yuan, Lin-Jing Zhong, Zhi-Min Wen, Zhe-Sheng Deng, Jian-Ming Liang, Rong-Xin Zheng, Min Article The selection of vascular grafts for coronary artery bypass surgery is crucial for a positive outcome. This study aimed to establish a novel line of vascular endothelial cells with a potent anticoagulant effect. A lentiviral vector was used to stably transfect human umbilical vein endothelial cells (HUVECs) with PGI2S alone (HUVEC-PGI2S) or both PGI2S and tPA (HUVEC-PGI2S-tPA). Both HUVEC-PGI2S and HUVEC-PGI2S-tPA cells over-expressing PGI2S and tPA were compared to mock-transfected cells. The enzyme-linked immuno sorbent assay (ELISAs) demonstrated that the anticoagulation components, ATIII and PLG, were up-regulated and coagulation factor FVIII was down-regulated in both cell lines. QRT-PCR and western blotting demonstrated the vasodilation and platelet disaggregation proteins PKA, PKC, and PTGIR were up-regulated in both cell lines, but MAPK expression was not altered in either cell line. However, cell viability and colony formation assays and cell cycle analysis demonstrated that both cell lines had a lower rate of cell growth and induced G1 phase arrest. HUVEC-PGI2S and HUVEC-PGI2S-tPA cells have a potent anticoagulant effect and their use in vascular heterografts may decrease the risk of thrombosis. Molecular Diversity Preservation International (MDPI) 2014-02-19 /pmc/articles/PMC3958884/ /pubmed/24557578 http://dx.doi.org/10.3390/ijms15022826 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wang, Jian-Hua Yuan, Lin-Jing Zhong, Zhi-Min Wen, Zhe-Sheng Deng, Jian-Ming Liang, Rong-Xin Zheng, Min
spellingShingle	Wang, Jian-Hua Yuan, Lin-Jing Zhong, Zhi-Min Wen, Zhe-Sheng Deng, Jian-Ming Liang, Rong-Xin Zheng, Min The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
author_facet	Wang, Jian-Hua Yuan, Lin-Jing Zhong, Zhi-Min Wen, Zhe-Sheng Deng, Jian-Ming Liang, Rong-Xin Zheng, Min
author_sort	Wang, Jian-Hua
title	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
title_short	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
title_full	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
title_fullStr	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
title_full_unstemmed	The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
title_sort	anticoagulant effect of pgi2s and tpa in transgenic umbilical vein endothelial cells is linked to up-regulation of pka and pkc
description	The selection of vascular grafts for coronary artery bypass surgery is crucial for a positive outcome. This study aimed to establish a novel line of vascular endothelial cells with a potent anticoagulant effect. A lentiviral vector was used to stably transfect human umbilical vein endothelial cells (HUVECs) with PGI2S alone (HUVEC-PGI2S) or both PGI2S and tPA (HUVEC-PGI2S-tPA). Both HUVEC-PGI2S and HUVEC-PGI2S-tPA cells over-expressing PGI2S and tPA were compared to mock-transfected cells. The enzyme-linked immuno sorbent assay (ELISAs) demonstrated that the anticoagulation components, ATIII and PLG, were up-regulated and coagulation factor FVIII was down-regulated in both cell lines. QRT-PCR and western blotting demonstrated the vasodilation and platelet disaggregation proteins PKA, PKC, and PTGIR were up-regulated in both cell lines, but MAPK expression was not altered in either cell line. However, cell viability and colony formation assays and cell cycle analysis demonstrated that both cell lines had a lower rate of cell growth and induced G1 phase arrest. HUVEC-PGI2S and HUVEC-PGI2S-tPA cells have a potent anticoagulant effect and their use in vascular heterografts may decrease the risk of thrombosis.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958884/
_version_	1612069170475696128

The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC

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