Ectopically tethered CP190 induces large-scale chromatin decondensation

Insulator mediated alteration in higher-order chromatin and/or nucleosome organization is an important aspect of epigenetic gene regulation. Recent studies have suggested a key role for CP190 in such processes. In this study, we analysed the effects of ectopically tethered insulator factors on chrom...

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Main Authors:	Ahanger, Sajad H., Günther, Katharina, Weth, Oliver, Bartkuhn, Marek, Bhonde, Ramesh R., Shouche, Yogesh S., Renkawitz, Rainer
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905270/

id	pubmed-3905270
recordtype	oai_dc
spelling	pubmed-39052702014-01-29 Ectopically tethered CP190 induces large-scale chromatin decondensation Ahanger, Sajad H. Günther, Katharina Weth, Oliver Bartkuhn, Marek Bhonde, Ramesh R. Shouche, Yogesh S. Renkawitz, Rainer Article Insulator mediated alteration in higher-order chromatin and/or nucleosome organization is an important aspect of epigenetic gene regulation. Recent studies have suggested a key role for CP190 in such processes. In this study, we analysed the effects of ectopically tethered insulator factors on chromatin structure and found that CP190 induces large-scale decondensation when targeted to a condensed lacO array in mammalian and Drosophila cells. In contrast, dCTCF alone, is unable to cause such a decondensation, however, when CP190 is present, dCTCF recruits it to the lacO array and mediates chromatin unfolding. The CP190 induced opening of chromatin may not be correlated with transcriptional activation, as binding of CP190 does not enhance luciferase activity in reporter assays. We propose that CP190 may mediate histone modification and chromatin remodelling activity to induce an open chromatin state by its direct recruitment or targeting by a DNA binding factor such as dCTCF. Nature Publishing Group 2014-01-29 /pmc/articles/PMC3905270/ /pubmed/24472778 http://dx.doi.org/10.1038/srep03917 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ahanger, Sajad H. Günther, Katharina Weth, Oliver Bartkuhn, Marek Bhonde, Ramesh R. Shouche, Yogesh S. Renkawitz, Rainer
spellingShingle	Ahanger, Sajad H. Günther, Katharina Weth, Oliver Bartkuhn, Marek Bhonde, Ramesh R. Shouche, Yogesh S. Renkawitz, Rainer Ectopically tethered CP190 induces large-scale chromatin decondensation
author_facet	Ahanger, Sajad H. Günther, Katharina Weth, Oliver Bartkuhn, Marek Bhonde, Ramesh R. Shouche, Yogesh S. Renkawitz, Rainer
author_sort	Ahanger, Sajad H.
title	Ectopically tethered CP190 induces large-scale chromatin decondensation
title_short	Ectopically tethered CP190 induces large-scale chromatin decondensation
title_full	Ectopically tethered CP190 induces large-scale chromatin decondensation
title_fullStr	Ectopically tethered CP190 induces large-scale chromatin decondensation
title_full_unstemmed	Ectopically tethered CP190 induces large-scale chromatin decondensation
title_sort	ectopically tethered cp190 induces large-scale chromatin decondensation
description	Insulator mediated alteration in higher-order chromatin and/or nucleosome organization is an important aspect of epigenetic gene regulation. Recent studies have suggested a key role for CP190 in such processes. In this study, we analysed the effects of ectopically tethered insulator factors on chromatin structure and found that CP190 induces large-scale decondensation when targeted to a condensed lacO array in mammalian and Drosophila cells. In contrast, dCTCF alone, is unable to cause such a decondensation, however, when CP190 is present, dCTCF recruits it to the lacO array and mediates chromatin unfolding. The CP190 induced opening of chromatin may not be correlated with transcriptional activation, as binding of CP190 does not enhance luciferase activity in reporter assays. We propose that CP190 may mediate histone modification and chromatin remodelling activity to induce an open chromatin state by its direct recruitment or targeting by a DNA binding factor such as dCTCF.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905270/
_version_	1612052270874099712

Ectopically tethered CP190 induces large-scale chromatin decondensation

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