Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects

Bacterial adherence to the acquired dental pellicle, important in dental caries (caries), is mediated by receptor-adhesins such as salivary agglutinin binding to Streptococcus mutans antigen I/II (I/II). Ten selected I/II epitopes were chosen to determine their reactivity to human salivary IgA. Prev...

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Main Authors:	McCarlie, V. Wallace, Hartsfield, James K., Blum, Janice S., González-Cabezas, Carlos, Chin, Judith R., Eckert, George J., Morford, Lorri A., Pescovitz, Mark D., Rodriguez, Henry, Fontana, Margherita, Gregory, Richard L.
Format:	Online
Language:	English
Published:	2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875298/

id	pubmed-3875298
recordtype	oai_dc
spelling	pubmed-38752982014-03-01 Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB104 positive subjects McCarlie, V. Wallace Hartsfield, James K. Blum, Janice S. González-Cabezas, Carlos Chin, Judith R. Eckert, George J. Morford, Lorri A. Pescovitz, Mark D. Rodriguez, Henry Fontana, Margherita Gregory, Richard L. Article Bacterial adherence to the acquired dental pellicle, important in dental caries (caries), is mediated by receptor-adhesins such as salivary agglutinin binding to Streptococcus mutans antigen I/II (I/II). Ten selected I/II epitopes were chosen to determine their reactivity to human salivary IgA. Previous studies suggested that a specific HLA biomarker group (HLA-DRB104) may have differential influence of immune responses to I/II. However, it was not known whether secretory IgA (SIgA) responses to the selected epitopes from HLA-DRB104 positive subjects were different compared to controls, or across other caries-related factors such as total IgA (TIgA). Thirty-two total subjects were matched according to HLA type, gender, ethnicity and age. HLA genotyping, oral bacterial, immunoglobulin and antibody analyses were performed. A large observed difference emerged with regard to the natural immune reservoir of TIgA in HLA-DRB104 positive subjects, specifically, a 27.6% reduction compared to controls. In contrast to all other epitopes studied, HLA-DRB104 positive subjects also exhibited reduced reactivity to I/II epitope 834–853. HLA-DRB104 positive subjects exhibited lower specific SIgA activity/TIgA to 834–853 and also a lower specific reactivity to 834–853/whole cell S. mutans UA159. Furthermore, HLA-DRB104 positive subjects exhibited lower responses to I/II in its entirety. The large observed difference in TIgA and the 834–853 reactivity pattern across multiple measures suggest potentially important connections pertaining to the link between HLA-DRB104 and caries. 2013-09-01 /pmc/articles/PMC3875298/ /pubmed/24386612 http://dx.doi.org/10.4236/oji.2013.33012 Text en Copyright © 2013 V. Wallace McCarlie et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	McCarlie, V. Wallace Hartsfield, James K. Blum, Janice S. González-Cabezas, Carlos Chin, Judith R. Eckert, George J. Morford, Lorri A. Pescovitz, Mark D. Rodriguez, Henry Fontana, Margherita Gregory, Richard L.
spellingShingle	McCarlie, V. Wallace Hartsfield, James K. Blum, Janice S. González-Cabezas, Carlos Chin, Judith R. Eckert, George J. Morford, Lorri A. Pescovitz, Mark D. Rodriguez, Henry Fontana, Margherita Gregory, Richard L. Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects
author_facet	McCarlie, V. Wallace Hartsfield, James K. Blum, Janice S. González-Cabezas, Carlos Chin, Judith R. Eckert, George J. Morford, Lorri A. Pescovitz, Mark D. Rodriguez, Henry Fontana, Margherita Gregory, Richard L.
author_sort	McCarlie, V. Wallace
title	Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects
title_short	Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects
title_full	Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects
title_fullStr	Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects
title_full_unstemmed	Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects
title_sort	total iga and iga reactivity to antigen i/ii epitopes in hla-drb1*04 positive subjects
description	Bacterial adherence to the acquired dental pellicle, important in dental caries (caries), is mediated by receptor-adhesins such as salivary agglutinin binding to Streptococcus mutans antigen I/II (I/II). Ten selected I/II epitopes were chosen to determine their reactivity to human salivary IgA. Previous studies suggested that a specific HLA biomarker group (HLA-DRB104) may have differential influence of immune responses to I/II. However, it was not known whether secretory IgA (SIgA) responses to the selected epitopes from HLA-DRB104 positive subjects were different compared to controls, or across other caries-related factors such as total IgA (TIgA). Thirty-two total subjects were matched according to HLA type, gender, ethnicity and age. HLA genotyping, oral bacterial, immunoglobulin and antibody analyses were performed. A large observed difference emerged with regard to the natural immune reservoir of TIgA in HLA-DRB104 positive subjects, specifically, a 27.6% reduction compared to controls. In contrast to all other epitopes studied, HLA-DRB104 positive subjects also exhibited reduced reactivity to I/II epitope 834–853. HLA-DRB104 positive subjects exhibited lower specific SIgA activity/TIgA to 834–853 and also a lower specific reactivity to 834–853/whole cell S. mutans UA159. Furthermore, HLA-DRB104 positive subjects exhibited lower responses to I/II in its entirety. The large observed difference in TIgA and the 834–853 reactivity pattern across multiple measures suggest potentially important connections pertaining to the link between HLA-DRB1*04 and caries.
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875298/
_version_	1612042266052919296

Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects

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