Human snRNA genes use polyadenylation factors to promote efficient transcription termination

RNA polymerase II transcribes both protein coding and non-coding RNA genes and, in yeast, different mechanisms terminate transcription of the two gene types. Transcription termination of mRNA genes is intricately coupled to cleavage and polyadenylation, whereas transcription of small nucleolar (sno)...

Full description

Bibliographic Details
Main Authors:	O’Reilly, Dawn, Kuznetsova, Olga V., Laitem, Clelia, Zaborowska, Justyna, Dienstbier, Martin, Murphy, Shona
Format:	Online
Language:	English
Published:	Oxford University Press 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874203/

id	pubmed-3874203
recordtype	oai_dc
spelling	pubmed-38742032013-12-28 Human snRNA genes use polyadenylation factors to promote efficient transcription termination O’Reilly, Dawn Kuznetsova, Olga V. Laitem, Clelia Zaborowska, Justyna Dienstbier, Martin Murphy, Shona Gene Regulation, Chromatin and Epigenetics RNA polymerase II transcribes both protein coding and non-coding RNA genes and, in yeast, different mechanisms terminate transcription of the two gene types. Transcription termination of mRNA genes is intricately coupled to cleavage and polyadenylation, whereas transcription of small nucleolar (sno)/small nuclear (sn)RNA genes is terminated by the RNA-binding proteins Nrd1, Nab3 and Sen1. The existence of an Nrd1-like pathway in humans has not yet been demonstrated. Using the U1 and U2 genes as models, we show that human snRNA genes are more similar to mRNA genes than yeast snRNA genes with respect to termination. The Integrator complex substitutes for the mRNA cleavage and polyadenylation specificity factor complex to promote cleavage and couple snRNA 3′-end processing with termination. Moreover, members of the associated with Pta1 (APT) and cleavage factor I/II complexes function as transcription terminators for human snRNA genes with little, if any, role in snRNA 3′-end processing. The gene-specific factor, proximal sequence element-binding transcription factor (PTF), helps clear the U1 and U2 genes of nucleosomes, which provides an easy passage for pol II, and the negative elongation factor facilitates termination at the end of the genes where nucleosome levels increase. Thus, human snRNA genes may use chromatin structure as an additional mechanism to promote efficient transcription termination in vivo. Oxford University Press 2014-01-01 2013-10-04 /pmc/articles/PMC3874203/ /pubmed/24097444 http://dx.doi.org/10.1093/nar/gkt892 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	O’Reilly, Dawn Kuznetsova, Olga V. Laitem, Clelia Zaborowska, Justyna Dienstbier, Martin Murphy, Shona
spellingShingle	O’Reilly, Dawn Kuznetsova, Olga V. Laitem, Clelia Zaborowska, Justyna Dienstbier, Martin Murphy, Shona Human snRNA genes use polyadenylation factors to promote efficient transcription termination
author_facet	O’Reilly, Dawn Kuznetsova, Olga V. Laitem, Clelia Zaborowska, Justyna Dienstbier, Martin Murphy, Shona
author_sort	O’Reilly, Dawn
title	Human snRNA genes use polyadenylation factors to promote efficient transcription termination
title_short	Human snRNA genes use polyadenylation factors to promote efficient transcription termination
title_full	Human snRNA genes use polyadenylation factors to promote efficient transcription termination
title_fullStr	Human snRNA genes use polyadenylation factors to promote efficient transcription termination
title_full_unstemmed	Human snRNA genes use polyadenylation factors to promote efficient transcription termination
title_sort	human snrna genes use polyadenylation factors to promote efficient transcription termination
description	RNA polymerase II transcribes both protein coding and non-coding RNA genes and, in yeast, different mechanisms terminate transcription of the two gene types. Transcription termination of mRNA genes is intricately coupled to cleavage and polyadenylation, whereas transcription of small nucleolar (sno)/small nuclear (sn)RNA genes is terminated by the RNA-binding proteins Nrd1, Nab3 and Sen1. The existence of an Nrd1-like pathway in humans has not yet been demonstrated. Using the U1 and U2 genes as models, we show that human snRNA genes are more similar to mRNA genes than yeast snRNA genes with respect to termination. The Integrator complex substitutes for the mRNA cleavage and polyadenylation specificity factor complex to promote cleavage and couple snRNA 3′-end processing with termination. Moreover, members of the associated with Pta1 (APT) and cleavage factor I/II complexes function as transcription terminators for human snRNA genes with little, if any, role in snRNA 3′-end processing. The gene-specific factor, proximal sequence element-binding transcription factor (PTF), helps clear the U1 and U2 genes of nucleosomes, which provides an easy passage for pol II, and the negative elongation factor facilitates termination at the end of the genes where nucleosome levels increase. Thus, human snRNA genes may use chromatin structure as an additional mechanism to promote efficient transcription termination in vivo.
publisher	Oxford University Press
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874203/
_version_	1612041919681003520

Human snRNA genes use polyadenylation factors to promote efficient transcription termination

Similar Items