BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas

BTG (B-cell translocation gene) can inhibit cell proliferation, metastasis, and angiogenesis and regulate cell cycle progression and differentiation in a variety of cell types. We aimed to clarify the role of BTG1 in ovarian carcinogenesis and progression. A BTG1-expressing plasmid was transfected i...

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Main Authors:	Zhao, Yang, Gou, Wen-Feng, Chen, Shuo, Takano, Yasuo, Xiu, Yin-Ling, Zheng, Hua-Chuan
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821579/

id	pubmed-3821579
recordtype	oai_dc
spelling	pubmed-38215792013-11-11 BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas Zhao, Yang Gou, Wen-Feng Chen, Shuo Takano, Yasuo Xiu, Yin-Ling Zheng, Hua-Chuan Article BTG (B-cell translocation gene) can inhibit cell proliferation, metastasis, and angiogenesis and regulate cell cycle progression and differentiation in a variety of cell types. We aimed to clarify the role of BTG1 in ovarian carcinogenesis and progression. A BTG1-expressing plasmid was transfected into ovarian carcinoma cells and their phenotypes and related proteins were examined. BTG1 mRNA expression was detected in ovarian normal tissue (n = 17), ovarian benign tumors (n = 12), and ovarian carcinoma (n = 64) using real-time RT-PCR. Ectopic BTG1 expression resulted in lower growth rate, high cisplatin sensitivity, G1 arrest, apoptosis, and decreased migration and invasion. Phosphoinositide 3-kinase, protein kinase B, Bcl-xL, survivin, vascular endothelial growth factor, and matrix metalloproteinase-2 mRNA and protein expression was reduced in transfectants as compared to control cells. There was higher expression of BTG1 mRNA in normal tissue than in carcinoma tissue (p = 0.001) and in benign tumors than in carcinoma tissue (p = 0.027). BTG1 mRNA expression in International Federation of Gynecology and Obstetrics (FIGO) stage I/II ovarian carcinomas was higher than that in FIGO stage III/IV ovarian carcinomas (p = 0.038). Altered BTG1 expression might play a role in the pathogenesis and progression of ovarian carcinoma by modulating proliferation, migration, invasion, the cell cycle, and apoptosis. Molecular Diversity Preservation International (MDPI) 2013-09-27 /pmc/articles/PMC3821579/ /pubmed/24084718 http://dx.doi.org/10.3390/ijms141019670 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhao, Yang Gou, Wen-Feng Chen, Shuo Takano, Yasuo Xiu, Yin-Ling Zheng, Hua-Chuan
spellingShingle	Zhao, Yang Gou, Wen-Feng Chen, Shuo Takano, Yasuo Xiu, Yin-Ling Zheng, Hua-Chuan BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
author_facet	Zhao, Yang Gou, Wen-Feng Chen, Shuo Takano, Yasuo Xiu, Yin-Ling Zheng, Hua-Chuan
author_sort	Zhao, Yang
title	BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
title_short	BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
title_full	BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
title_fullStr	BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
title_full_unstemmed	BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
title_sort	btg1 expression correlates with the pathogenesis and progression of ovarian carcinomas
description	BTG (B-cell translocation gene) can inhibit cell proliferation, metastasis, and angiogenesis and regulate cell cycle progression and differentiation in a variety of cell types. We aimed to clarify the role of BTG1 in ovarian carcinogenesis and progression. A BTG1-expressing plasmid was transfected into ovarian carcinoma cells and their phenotypes and related proteins were examined. BTG1 mRNA expression was detected in ovarian normal tissue (n = 17), ovarian benign tumors (n = 12), and ovarian carcinoma (n = 64) using real-time RT-PCR. Ectopic BTG1 expression resulted in lower growth rate, high cisplatin sensitivity, G1 arrest, apoptosis, and decreased migration and invasion. Phosphoinositide 3-kinase, protein kinase B, Bcl-xL, survivin, vascular endothelial growth factor, and matrix metalloproteinase-2 mRNA and protein expression was reduced in transfectants as compared to control cells. There was higher expression of BTG1 mRNA in normal tissue than in carcinoma tissue (p = 0.001) and in benign tumors than in carcinoma tissue (p = 0.027). BTG1 mRNA expression in International Federation of Gynecology and Obstetrics (FIGO) stage I/II ovarian carcinomas was higher than that in FIGO stage III/IV ovarian carcinomas (p = 0.038). Altered BTG1 expression might play a role in the pathogenesis and progression of ovarian carcinoma by modulating proliferation, migration, invasion, the cell cycle, and apoptosis.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821579/
_version_	1612024763237007360

BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas

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