Linking genotype to phenotype on beads: high throughput selection of peptides with biological function

Linking genotype to phenotype on beads: high throughput selection of peptides with biological function

Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity an...

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Main Authors: Huang, Li-Chieh, Pan, Xiaoyan, Yang, Hongbing, Wan, Lai Kin Derek, Stewart-Jones, Guillaume, Dorrell, Lucy, Ogg, Graham
Format: Online
Language:English
Published: Nature Publishing Group 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805977/
id pubmed-3805977
recordtype oai_dc
spelling pubmed-38059772013-10-23 Linking genotype to phenotype on beads: high throughput selection of peptides with biological function Huang, Li-Chieh Pan, Xiaoyan Yang, Hongbing Wan, Lai Kin Derek Stewart-Jones, Guillaume Dorrell, Lucy Ogg, Graham Article Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro through blocking of protein:protein interaction with the co-receptor CCR5. The bead display approach has many potential applications for probing biological systems and for drug lead development. Nature Publishing Group 2013-10-23 /pmc/articles/PMC3805977/ /pubmed/24149829 http://dx.doi.org/10.1038/srep03030 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-Non-Commercial-ShareAlike 3.0 Unported licence. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Huang, Li-Chieh
Pan, Xiaoyan
Yang, Hongbing
Wan, Lai Kin Derek
Stewart-Jones, Guillaume
Dorrell, Lucy
Ogg, Graham
spellingShingle Huang, Li-Chieh
Pan, Xiaoyan
Yang, Hongbing
Wan, Lai Kin Derek
Stewart-Jones, Guillaume
Dorrell, Lucy
Ogg, Graham
Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
author_facet Huang, Li-Chieh
Pan, Xiaoyan
Yang, Hongbing
Wan, Lai Kin Derek
Stewart-Jones, Guillaume
Dorrell, Lucy
Ogg, Graham
author_sort Huang, Li-Chieh
title Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
title_short Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
title_full Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
title_fullStr Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
title_full_unstemmed Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
title_sort linking genotype to phenotype on beads: high throughput selection of peptides with biological function
description Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro through blocking of protein:protein interaction with the co-receptor CCR5. The bead display approach has many potential applications for probing biological systems and for drug lead development.
publisher Nature Publishing Group
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805977/
_version_ 1612020157019848704

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