Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae

Silica nanoparticles (SiNPs) have been widely used in biomedical and biotechnological applications. Environmental exposure to nanomaterials is inevitable as they become part of our daily life. Therefore, it is necessary to investigate the possible toxic effects of SiNPs exposure. In this study, zebr...

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Main Authors:	Duan, Junchao, Yu, Yongbo, Shi, Huiqin, Tian, Linwei, Guo, Caixia, Huang, Peili, Zhou, Xianqing, Peng, Shuangqing, Sun, Zhiwei
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776836/

id	pubmed-3776836
recordtype	oai_dc
spelling	pubmed-37768362013-09-20 Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae Duan, Junchao Yu, Yongbo Shi, Huiqin Tian, Linwei Guo, Caixia Huang, Peili Zhou, Xianqing Peng, Shuangqing Sun, Zhiwei Research Article Silica nanoparticles (SiNPs) have been widely used in biomedical and biotechnological applications. Environmental exposure to nanomaterials is inevitable as they become part of our daily life. Therefore, it is necessary to investigate the possible toxic effects of SiNPs exposure. In this study, zebrafish embryos were treated with SiNPs (25, 50, 100, 200 µg/mL) during 4–96 hours post fertilization (hpf). Mortality, hatching rate, malformation and whole-embryo cellular death were detected. We also measured the larval behavior to analyze whether SiNPs had adverse effects on larvae locomotor activity. The results showed that as the exposure dosages increasing, the hatching rate of zebrafish embryos was decreased while the mortality and cell death were increased. Exposure to SiNPs caused embryonic malformations, including pericardial edema, yolk sac edema, tail and head malformation. The larval behavior testing showed that the total swimming distance was decreased in a dose-dependent manner. The lower dose (25 and 50 µg/mL SiNPs) produced substantial hyperactivity while the higher doses (100 and 200 µg/mL SiNPs) elicited remarkably hypoactivity in dark periods. In summary, our data indicated that SiNPs caused embryonic developmental toxicity, resulted in persistent effects on larval behavior. Public Library of Science 2013-09-18 /pmc/articles/PMC3776836/ /pubmed/24058598 http://dx.doi.org/10.1371/journal.pone.0074606 Text en © 2013 Duan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Duan, Junchao Yu, Yongbo Shi, Huiqin Tian, Linwei Guo, Caixia Huang, Peili Zhou, Xianqing Peng, Shuangqing Sun, Zhiwei
spellingShingle	Duan, Junchao Yu, Yongbo Shi, Huiqin Tian, Linwei Guo, Caixia Huang, Peili Zhou, Xianqing Peng, Shuangqing Sun, Zhiwei Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae
author_facet	Duan, Junchao Yu, Yongbo Shi, Huiqin Tian, Linwei Guo, Caixia Huang, Peili Zhou, Xianqing Peng, Shuangqing Sun, Zhiwei
author_sort	Duan, Junchao
title	Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae
title_short	Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae
title_full	Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae
title_fullStr	Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae
title_full_unstemmed	Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae
title_sort	toxic effects of silica nanoparticles on zebrafish embryos and larvae
description	Silica nanoparticles (SiNPs) have been widely used in biomedical and biotechnological applications. Environmental exposure to nanomaterials is inevitable as they become part of our daily life. Therefore, it is necessary to investigate the possible toxic effects of SiNPs exposure. In this study, zebrafish embryos were treated with SiNPs (25, 50, 100, 200 µg/mL) during 4–96 hours post fertilization (hpf). Mortality, hatching rate, malformation and whole-embryo cellular death were detected. We also measured the larval behavior to analyze whether SiNPs had adverse effects on larvae locomotor activity. The results showed that as the exposure dosages increasing, the hatching rate of zebrafish embryos was decreased while the mortality and cell death were increased. Exposure to SiNPs caused embryonic malformations, including pericardial edema, yolk sac edema, tail and head malformation. The larval behavior testing showed that the total swimming distance was decreased in a dose-dependent manner. The lower dose (25 and 50 µg/mL SiNPs) produced substantial hyperactivity while the higher doses (100 and 200 µg/mL SiNPs) elicited remarkably hypoactivity in dark periods. In summary, our data indicated that SiNPs caused embryonic developmental toxicity, resulted in persistent effects on larval behavior.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776836/
_version_	1612012577252966400

Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae

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