Comparison of In Vitro and In Situ Methods for Studying Lipolysis
Lipolysis is a highly regulated process and is controlled by nervous system, hormones, and paracrine/autocrine factors. Dysregulation of lipolysis is associated with some pathophysiological conditions including diabetes, metabolic syndrome, and obesity. Nowadays, special attention isthereforepaid to...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760109/ |
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pubmed-37601092013-09-10 Comparison of In Vitro and In Situ Methods for Studying Lipolysis Ghorbani, Ahmad Abedinzade, Mahmood Review Article Lipolysis is a highly regulated process and is controlled by nervous system, hormones, and paracrine/autocrine factors. Dysregulation of lipolysis is associated with some pathophysiological conditions including diabetes, metabolic syndrome, and obesity. Nowadays, special attention isthereforepaid to study lipolysis using different experimental models. This review summarizes the current experimental methods for studying lipolysis. Culture of preadipocyte cell lines, use of differentiated stroma-vascular cells, primary culture of adipocyte, organ culture of adipose tissue, and microdialysis technique are the most widely used techniques to study lipolysis. The advantages and limitations of using these methods are discussed. Hindawi Publishing Corporation 2013-08-19 /pmc/articles/PMC3760109/ /pubmed/24024037 http://dx.doi.org/10.1155/2013/205385 Text en Copyright © 2013 A. Ghorbani and M. Abedinzade. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ghorbani, Ahmad Abedinzade, Mahmood |
| spellingShingle |
Ghorbani, Ahmad Abedinzade, Mahmood Comparison of In Vitro and In Situ Methods for Studying Lipolysis |
| author_facet |
Ghorbani, Ahmad Abedinzade, Mahmood |
| author_sort |
Ghorbani, Ahmad |
| title |
Comparison of In Vitro and In Situ Methods for Studying Lipolysis |
| title_short |
Comparison of In Vitro and In Situ Methods for Studying Lipolysis |
| title_full |
Comparison of In Vitro and In Situ Methods for Studying Lipolysis |
| title_fullStr |
Comparison of In Vitro and In Situ Methods for Studying Lipolysis |
| title_full_unstemmed |
Comparison of In Vitro and In Situ Methods for Studying Lipolysis |
| title_sort |
comparison of in vitro and in situ methods for studying lipolysis |
| description |
Lipolysis is a highly regulated process and is controlled by nervous system, hormones, and paracrine/autocrine factors. Dysregulation of lipolysis is associated with some pathophysiological conditions including diabetes, metabolic syndrome, and obesity. Nowadays, special attention isthereforepaid to study lipolysis using different experimental models. This review summarizes the current experimental methods for studying lipolysis. Culture of preadipocyte cell lines, use of differentiated stroma-vascular cells, primary culture of adipocyte, organ culture of adipose tissue, and microdialysis technique are the most widely used techniques to study lipolysis. The advantages and limitations of using these methods are discussed. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760109/ |
| _version_ |
1612007794281545728 |