ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo

ECRG4 has been shown to be a candidate tumor suppressor in several tumors, but its role in head and neck cancer remains poorly understood. In the present study, the effect of ECRG4 on head and neck cancer was investigated in vitro and in vivo. pFLAG-CMV-2-ECRG4 was stably transfected into squamous c...

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Main Authors:	XU, TING, XIAO, DAJIANG, ZHANG, XIN
Format:	Online
Language:	English
Published:	D.A. Spandidos 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700983/

id	pubmed-3700983
recordtype	oai_dc
spelling	pubmed-37009832013-07-05 ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo XU, TING XIAO, DAJIANG ZHANG, XIN Articles ECRG4 has been shown to be a candidate tumor suppressor in several tumors, but its role in head and neck cancer remains poorly understood. In the present study, the effect of ECRG4 on head and neck cancer was investigated in vitro and in vivo. pFLAG-CMV-2-ECRG4 was stably transfected into squamous cell carcinoma of the head and neck (SCCHN) M2 cell lines to overexpress the ECRG4 gene. Real-time PCR and western blot analysis were performed to detect gene and protein expression, respectively. An MTT assay and flow cytometric analysis were used to detect the growth of M2 cells. Matrigel™ invasion and scratch assays were applied to observe the invasion and migration of the cells. A tumorigenicity assay was applied to test the tumor growth and cervical lymph node metastasis in vivo. Based on the data, pFLAG-CMV-2-ECRG4 significantly increased the expression of ECRG4 in the M2 cells. The constructed plasmid inhibited cell proliferation and promoted cell cycle arrest and apoptosis in the M2 cells. The growth rate and metastasis of the tumor cells in xenografts were suppressed following the overexpression of ECRG4 in nude mice. These data suggest that ECRG4 plays a significant role in the regulation of growth and metastasis in SCCHN, providing new clues for the diagnosis and therapy of SCCHN. D.A. Spandidos 2013-06 2013-04-10 /pmc/articles/PMC3700983/ /pubmed/23833667 http://dx.doi.org/10.3892/ol.2013.1298 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	XU, TING XIAO, DAJIANG ZHANG, XIN
spellingShingle	XU, TING XIAO, DAJIANG ZHANG, XIN ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
author_facet	XU, TING XIAO, DAJIANG ZHANG, XIN
author_sort	XU, TING
title	ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
title_short	ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
title_full	ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
title_fullStr	ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
title_full_unstemmed	ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
title_sort	ecrg4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo
description	ECRG4 has been shown to be a candidate tumor suppressor in several tumors, but its role in head and neck cancer remains poorly understood. In the present study, the effect of ECRG4 on head and neck cancer was investigated in vitro and in vivo. pFLAG-CMV-2-ECRG4 was stably transfected into squamous cell carcinoma of the head and neck (SCCHN) M2 cell lines to overexpress the ECRG4 gene. Real-time PCR and western blot analysis were performed to detect gene and protein expression, respectively. An MTT assay and flow cytometric analysis were used to detect the growth of M2 cells. Matrigel™ invasion and scratch assays were applied to observe the invasion and migration of the cells. A tumorigenicity assay was applied to test the tumor growth and cervical lymph node metastasis in vivo. Based on the data, pFLAG-CMV-2-ECRG4 significantly increased the expression of ECRG4 in the M2 cells. The constructed plasmid inhibited cell proliferation and promoted cell cycle arrest and apoptosis in the M2 cells. The growth rate and metastasis of the tumor cells in xenografts were suppressed following the overexpression of ECRG4 in nude mice. These data suggest that ECRG4 plays a significant role in the regulation of growth and metastasis in SCCHN, providing new clues for the diagnosis and therapy of SCCHN.
publisher	D.A. Spandidos
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3700983/
_version_	1611991648220217344

ECRG4 inhibits growth and invasiveness of squamous cell carcinoma of the head and neck in vitro and in vivo

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