Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★

Poor prenatal nutrition, acting through epigenetic processes, induces persistent changes in offspring phenotype. We investigated the effect of maternal fat intake on polyunsaturated fatty acid (PUFA) status and on the epigenetic regulation of Fads2, encoding Δ6 desaturase (rate limiting in PUFA synt...

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Main Authors:	Hoile, Samuel P., Irvine, Nicola A., Kelsall, Christopher J., Sibbons, Charlene, Feunteun, Aurélie, Collister, Alex, Torrens, Christopher, Calder, Philip C., Hanson, Mark A., Lillycrop, Karen A., Burdge, Graham C.
Format:	Online
Language:	English
Published:	Elsevier Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698442/

id	pubmed-3698442
recordtype	oai_dc
spelling	pubmed-36984422013-07-02 Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★ Hoile, Samuel P. Irvine, Nicola A. Kelsall, Christopher J. Sibbons, Charlene Feunteun, Aurélie Collister, Alex Torrens, Christopher Calder, Philip C. Hanson, Mark A. Lillycrop, Karen A. Burdge, Graham C. Research Article Poor prenatal nutrition, acting through epigenetic processes, induces persistent changes in offspring phenotype. We investigated the effect of maternal fat intake on polyunsaturated fatty acid (PUFA) status and on the epigenetic regulation of Fads2, encoding Δ6 desaturase (rate limiting in PUFA synthesis), in the adult offspring. Rats (n=6 per dietary group) were fed either 3.5% (w/w), 7% (w/w) or 21% (w/w) butter or fish oil (FO) from 14 days preconception until weaning. Offspring (n=6 males and females per dietary group) were fed 4% (w/w) soybean oil until postnatal day 77. 20:4n-6 and 22:6n-3 levels were lower in liver phosphatidylcholine (PC) and phosphatidylethanolamine and plasma PC (all P<.0001) in offspring of dams fed 21% than 3.5% or 7% fat regardless of type. Hepatic Fads2 expression related inversely to maternal dietary fat. Fads2 messenger RNA expression correlated negatively with methylation of CpGs at −623, −394, −84 and −76 bases relative to the transcription start site (all P<.005). Methylation of these CpGs was higher in offspring of dams fed 21% than 3.5% or 7% fat; FO higher than butter. Feeding adult female rats 7% fat reduced 20:4n-6 status in liver PC and Fads2 expression and increased methylation of CpGs −623, −394, −84 and −76 that reversed in animals switched from 7% to 4% fat diets. These findings suggest that fat exposure during development induces persistent changes, while adults exhibit a transient response, in hepatic PUFA status in offspring through epigenetic regulation of Fads2. Thus, epigenetic regulation of Fads2 may contribute to short- and long-term regulation of PUFA synthesis. Elsevier Science 2013-07 /pmc/articles/PMC3698442/ /pubmed/23107313 http://dx.doi.org/10.1016/j.jnutbio.2012.09.005 Text en © 2013 Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hoile, Samuel P. Irvine, Nicola A. Kelsall, Christopher J. Sibbons, Charlene Feunteun, Aurélie Collister, Alex Torrens, Christopher Calder, Philip C. Hanson, Mark A. Lillycrop, Karen A. Burdge, Graham C.
spellingShingle	Hoile, Samuel P. Irvine, Nicola A. Kelsall, Christopher J. Sibbons, Charlene Feunteun, Aurélie Collister, Alex Torrens, Christopher Calder, Philip C. Hanson, Mark A. Lillycrop, Karen A. Burdge, Graham C. Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★
author_facet	Hoile, Samuel P. Irvine, Nicola A. Kelsall, Christopher J. Sibbons, Charlene Feunteun, Aurélie Collister, Alex Torrens, Christopher Calder, Philip C. Hanson, Mark A. Lillycrop, Karen A. Burdge, Graham C.
author_sort	Hoile, Samuel P.
title	Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★
title_short	Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★
title_full	Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★
title_fullStr	Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★
title_full_unstemmed	Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★
title_sort	maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of fads2 in offspring liver☆☆☆★
description	Poor prenatal nutrition, acting through epigenetic processes, induces persistent changes in offspring phenotype. We investigated the effect of maternal fat intake on polyunsaturated fatty acid (PUFA) status and on the epigenetic regulation of Fads2, encoding Δ6 desaturase (rate limiting in PUFA synthesis), in the adult offspring. Rats (n=6 per dietary group) were fed either 3.5% (w/w), 7% (w/w) or 21% (w/w) butter or fish oil (FO) from 14 days preconception until weaning. Offspring (n=6 males and females per dietary group) were fed 4% (w/w) soybean oil until postnatal day 77. 20:4n-6 and 22:6n-3 levels were lower in liver phosphatidylcholine (PC) and phosphatidylethanolamine and plasma PC (all P<.0001) in offspring of dams fed 21% than 3.5% or 7% fat regardless of type. Hepatic Fads2 expression related inversely to maternal dietary fat. Fads2 messenger RNA expression correlated negatively with methylation of CpGs at −623, −394, −84 and −76 bases relative to the transcription start site (all P<.005). Methylation of these CpGs was higher in offspring of dams fed 21% than 3.5% or 7% fat; FO higher than butter. Feeding adult female rats 7% fat reduced 20:4n-6 status in liver PC and Fads2 expression and increased methylation of CpGs −623, −394, −84 and −76 that reversed in animals switched from 7% to 4% fat diets. These findings suggest that fat exposure during development induces persistent changes, while adults exhibit a transient response, in hepatic PUFA status in offspring through epigenetic regulation of Fads2. Thus, epigenetic regulation of Fads2 may contribute to short- and long-term regulation of PUFA synthesis.
publisher	Elsevier Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698442/
_version_	1611990966027157504

Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver☆☆☆★

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