Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development

Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice. Methods. Chronic granulomatous infl...

Full description

Bibliographic Details
Main Authors:	Menshchikova, Elena, Zenkov, Nikolay, Tkachev, Victor, Potapova, Oksana, Cherdantseva, Liliya, Shkurupiy, Vyacheslav
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655644/

id	pubmed-3655644
recordtype	oai_dc
spelling	pubmed-36556442013-06-04 Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development Menshchikova, Elena Zenkov, Nikolay Tkachev, Victor Potapova, Oksana Cherdantseva, Liliya Shkurupiy, Vyacheslav Research Article Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice. Methods. Chronic granulomatous inflammation was induced in male BALB/c mice by intravenously injecting the BCG vaccine, and the production of oxidative stress (activity of free-radical oxidation processes) and histological changes in the lungs, liver, and peritoneal exudate were measured 3, 30, 60, and 90 days after infection. Results. The tuberculous granuloma numerical density and diameter continuously increased from day 30 to day 90, and the macrophage content within the granulomas progressively diminished with a concomitant elevation in the number of epithelioid cells. The activity of the free-radical oxidation processes in the liver (i.e., the intensity of the homogenate chemiluminescence) reached a maximum at postinfection day 60 and subsequently began to decrease. The peak generation of reactive oxygen species by phagocytes in the peritoneal exudate (measured using flow cytometry) was also shifted in time and fell on day 30. Conclusions. The rise in the steady-state concentration of H2O2 in the liver of mice with BCG-induced granulomatosis is not related to local H2O2 production by phagocytes, and a decrease in the severity of generalized inflammation precedes the resolution of local inflammation. Hindawi Publishing Corporation 2013 2013-04-23 /pmc/articles/PMC3655644/ /pubmed/23738038 http://dx.doi.org/10.1155/2013/452546 Text en Copyright © 2013 Elena Menshchikova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Menshchikova, Elena Zenkov, Nikolay Tkachev, Victor Potapova, Oksana Cherdantseva, Liliya Shkurupiy, Vyacheslav
spellingShingle	Menshchikova, Elena Zenkov, Nikolay Tkachev, Victor Potapova, Oksana Cherdantseva, Liliya Shkurupiy, Vyacheslav Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development
author_facet	Menshchikova, Elena Zenkov, Nikolay Tkachev, Victor Potapova, Oksana Cherdantseva, Liliya Shkurupiy, Vyacheslav
author_sort	Menshchikova, Elena
title	Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development
title_short	Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development
title_full	Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development
title_fullStr	Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development
title_full_unstemmed	Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development
title_sort	oxidative stress and free-radical oxidation in bcg granulomatosis development
description	Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice. Methods. Chronic granulomatous inflammation was induced in male BALB/c mice by intravenously injecting the BCG vaccine, and the production of oxidative stress (activity of free-radical oxidation processes) and histological changes in the lungs, liver, and peritoneal exudate were measured 3, 30, 60, and 90 days after infection. Results. The tuberculous granuloma numerical density and diameter continuously increased from day 30 to day 90, and the macrophage content within the granulomas progressively diminished with a concomitant elevation in the number of epithelioid cells. The activity of the free-radical oxidation processes in the liver (i.e., the intensity of the homogenate chemiluminescence) reached a maximum at postinfection day 60 and subsequently began to decrease. The peak generation of reactive oxygen species by phagocytes in the peritoneal exudate (measured using flow cytometry) was also shifted in time and fell on day 30. Conclusions. The rise in the steady-state concentration of H2O2 in the liver of mice with BCG-induced granulomatosis is not related to local H2O2 production by phagocytes, and a decrease in the severity of generalized inflammation precedes the resolution of local inflammation.
publisher	Hindawi Publishing Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655644/
_version_	1611978316606078976

Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development

Similar Items