Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands

Rhythmic expression of period (per) and timeless (tim) genes in central circadian pacemaker neurons and prothoracic gland cells, part of the peripheral circadian oscillators in flies, may synergistically control eclosion rhythms, but their oscillatory profiles remain unclear. Here we show difference...

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Main Authors:	Morioka, Eri, Matsumoto, Akira, Ikeda, Masayuki
Format:	Online
Language:	English
Published:	Nature Pub. Group 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621432/

id	pubmed-3621432
recordtype	oai_dc
spelling	pubmed-36214322013-04-10 Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands Morioka, Eri Matsumoto, Akira Ikeda, Masayuki Article Rhythmic expression of period (per) and timeless (tim) genes in central circadian pacemaker neurons and prothoracic gland cells, part of the peripheral circadian oscillators in flies, may synergistically control eclosion rhythms, but their oscillatory profiles remain unclear. Here we show differences and interactions between peripheral and central oscillators using per-luciferase and cytosolic Ca2+ reporter (yellow cameleon) imaging in organotypic prothoracic gland cultures with or without the associated central nervous system. Isolated prothoracic gland cells exhibit light-insensitive synchronous per-transcriptional rhythms. In prothoracic gland cells associated with the central nervous system, however, per transcription is markedly amplified following 12-h light exposure, resulting in the manifestation of day–night rhythms in nuclear PER immunostaining levels and spontaneous Ca2+ spiking. Unlike PER expression, nuclear TIM expression is associated with day–night cycles that are independent of the central nervous system. These results demonstrate that photoreception and synaptic signal transduction in/from the central nervous system coordinate molecular 'gears' in endocrine oscillators to generate physiological rhythms. Nature Pub. Group 2012-06-19 /pmc/articles/PMC3621432/ /pubmed/22713751 http://dx.doi.org/10.1038/ncomms1922 Text en Copyright © 2012, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Morioka, Eri Matsumoto, Akira Ikeda, Masayuki
spellingShingle	Morioka, Eri Matsumoto, Akira Ikeda, Masayuki Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands
author_facet	Morioka, Eri Matsumoto, Akira Ikeda, Masayuki
author_sort	Morioka, Eri
title	Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands
title_short	Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands
title_full	Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands
title_fullStr	Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands
title_full_unstemmed	Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands
title_sort	neuronal influence on peripheral circadian oscillators in pupal drosophila prothoracic glands
description	Rhythmic expression of period (per) and timeless (tim) genes in central circadian pacemaker neurons and prothoracic gland cells, part of the peripheral circadian oscillators in flies, may synergistically control eclosion rhythms, but their oscillatory profiles remain unclear. Here we show differences and interactions between peripheral and central oscillators using per-luciferase and cytosolic Ca2+ reporter (yellow cameleon) imaging in organotypic prothoracic gland cultures with or without the associated central nervous system. Isolated prothoracic gland cells exhibit light-insensitive synchronous per-transcriptional rhythms. In prothoracic gland cells associated with the central nervous system, however, per transcription is markedly amplified following 12-h light exposure, resulting in the manifestation of day–night rhythms in nuclear PER immunostaining levels and spontaneous Ca2+ spiking. Unlike PER expression, nuclear TIM expression is associated with day–night cycles that are independent of the central nervous system. These results demonstrate that photoreception and synaptic signal transduction in/from the central nervous system coordinate molecular 'gears' in endocrine oscillators to generate physiological rhythms.
publisher	Nature Pub. Group
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621432/
_version_	1611968939798036480

Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands

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