Family-Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides
Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer software, for which the starting point is a mult...
Main Authors: | Iserte, Javier Alonso, Stephan, Betina Ines, Goñi, Sandra Elizabeth, Borio, Cristina Silvia, Ghiringhelli, Pablo Daniel, Lozano, Mario Enrique |
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Format: | Online |
Language: | English |
Published: |
Hindawi Publishing Corporation
2013
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600133/ |
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