Greene SCPrimer: a rapid comprehensive tool for designing degenerate primers from multiple sequence alignments
Polymerase chain reaction (PCR) is widely applied in clinical and environmental microbiology. Primer design is key to the development of successful assays and is often performed manually by using multiple nucleic acid alignments. Few public software tools exist that allow comprehensive design of deg...
Main Authors: | , , , , , , , |
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Format: | Online |
Language: | English |
Published: |
Oxford University Press
2006
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1747188/ |