Greene SCPrimer: a rapid comprehensive tool for designing degenerate primers from multiple sequence alignments

Polymerase chain reaction (PCR) is widely applied in clinical and environmental microbiology. Primer design is key to the development of successful assays and is often performed manually by using multiple nucleic acid alignments. Few public software tools exist that allow comprehensive design of deg...

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Bibliographic Details
Main Authors: Jabado, Omar J., Palacios, Gustavo, Kapoor, Vishal, Hui, Jeffrey, Renwick, Neil, Zhai, Junhui, Briese, Thomas, Lipkin, W. Ian
Format: Online
Language:English
Published: Oxford University Press 2006
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1747188/