Global analysis of mRNA stability in Mycobacterium tuberculosis
Mycobacterium tuberculosis (MTB) is a highly successful pathogen that infects over a billion people. As with most organisms, MTB adapts to stress by modifying its transcriptional profile. Remodeling of the transcriptome requires both altering the transcription rate and clearing away the existing mRN...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2013
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592478/ |
| id |
pubmed-3592478 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-35924782013-03-08 Global analysis of mRNA stability in Mycobacterium tuberculosis Rustad, Tige R. Minch, Kyle J. Brabant, William Winkler, Jessica K. Reiss, David J. Baliga, Nitin S. Sherman, David R. RNA Mycobacterium tuberculosis (MTB) is a highly successful pathogen that infects over a billion people. As with most organisms, MTB adapts to stress by modifying its transcriptional profile. Remodeling of the transcriptome requires both altering the transcription rate and clearing away the existing mRNA through degradation, a process that can be directly regulated in response to stress. To understand better how MTB adapts to the harsh environs of the human host, we performed a global survey of the decay rates of MTB mRNA transcripts. Decay rates were measured for 2139 of the ∼4000 MTB genes, which displayed an average half-life of 9.5 min. This is nearly twice the average mRNA half-life of other prokaryotic organisms where these measurements have been made. The transcriptome was further stabilized in response to lowered temperature and hypoxic stress. The generally stable transcriptome described here, and the additional stabilization in response to physiologically relevant stresses, has far-ranging implications for how this pathogen is able to adapt in its human host. Oxford University Press 2013-01 2012-11-02 /pmc/articles/PMC3592478/ /pubmed/23125364 http://dx.doi.org/10.1093/nar/gks1019 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Rustad, Tige R. Minch, Kyle J. Brabant, William Winkler, Jessica K. Reiss, David J. Baliga, Nitin S. Sherman, David R. |
| spellingShingle |
Rustad, Tige R. Minch, Kyle J. Brabant, William Winkler, Jessica K. Reiss, David J. Baliga, Nitin S. Sherman, David R. Global analysis of mRNA stability in Mycobacterium tuberculosis |
| author_facet |
Rustad, Tige R. Minch, Kyle J. Brabant, William Winkler, Jessica K. Reiss, David J. Baliga, Nitin S. Sherman, David R. |
| author_sort |
Rustad, Tige R. |
| title |
Global analysis of mRNA stability in Mycobacterium tuberculosis |
| title_short |
Global analysis of mRNA stability in Mycobacterium tuberculosis |
| title_full |
Global analysis of mRNA stability in Mycobacterium tuberculosis |
| title_fullStr |
Global analysis of mRNA stability in Mycobacterium tuberculosis |
| title_full_unstemmed |
Global analysis of mRNA stability in Mycobacterium tuberculosis |
| title_sort |
global analysis of mrna stability in mycobacterium tuberculosis |
| description |
Mycobacterium tuberculosis (MTB) is a highly successful pathogen that infects over a billion people. As with most organisms, MTB adapts to stress by modifying its transcriptional profile. Remodeling of the transcriptome requires both altering the transcription rate and clearing away the existing mRNA through degradation, a process that can be directly regulated in response to stress. To understand better how MTB adapts to the harsh environs of the human host, we performed a global survey of the decay rates of MTB mRNA transcripts. Decay rates were measured for 2139 of the ∼4000 MTB genes, which displayed an average half-life of 9.5 min. This is nearly twice the average mRNA half-life of other prokaryotic organisms where these measurements have been made. The transcriptome was further stabilized in response to lowered temperature and hypoxic stress. The generally stable transcriptome described here, and the additional stabilization in response to physiologically relevant stresses, has far-ranging implications for how this pathogen is able to adapt in its human host. |
| publisher |
Oxford University Press |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592478/ |
| _version_ |
1611960865361231872 |