Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model

Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to induce apoptosis in a variety of cancer cells, including colon, prostate, breast and leukemia. Among them, aspirin, a classical NSAID, shows promise in cancer therapy in certain types of cancers. We hypothesized that aspirin might affect the...

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Main Authors:	HOSSAIN, MOHAMMAD AKBAR, KIM, DONG HWAN, JANG, JUNG YOON, KANG, YONG JUNG, YOON, JEONG-HYUN, MOON, JEON-OK, CHUNG, HAE YOUNG, KIM, GI-YOUNG, CHOI, YUNG HYUN, COPPLE, BRYAN L., KIM, NAM DEUK
Format:	Online
Language:	English
Published:	D.A. Spandidos 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584583/

id	pubmed-3584583
recordtype	oai_dc
spelling	pubmed-35845832013-03-04 Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model HOSSAIN, MOHAMMAD AKBAR KIM, DONG HWAN JANG, JUNG YOON KANG, YONG JUNG YOON, JEONG-HYUN MOON, JEON-OK CHUNG, HAE YOUNG KIM, GI-YOUNG CHOI, YUNG HYUN COPPLE, BRYAN L. KIM, NAM DEUK Articles Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to induce apoptosis in a variety of cancer cells, including colon, prostate, breast and leukemia. Among them, aspirin, a classical NSAID, shows promise in cancer therapy in certain types of cancers. We hypothesized that aspirin might affect the growth of liver cancer cells since liver is the principal site for aspirin metabolism. Therefore, we investigated the effects of aspirin on the HepG2 human hepatocellular carcinoma cell line in vitro and the HepG2 cell xenograft model in BALB/c nude mice. We found that treatment with aspirin inhibited cell growth and induced apoptosis involving both extrinsic and intrinsic pathways as measured by DNA ladder formation, alteration in the Bax/Bcl-2 ratio, activation of the caspase activities and related protein expressions. In vivo antitumor activity assay also showed that aspirin resulted in significant tumor growth inhibition compared to the control. Oral administration of aspirin (100 mg/kg/day) caused a significant reduction in the growth of HepG2 tumors in nude mice. These findings suggest that aspirin may be used as a promising anticancer agent against liver cancer. D.A. Spandidos 2011-12-15 /pmc/articles/PMC3584583/ /pubmed/22179060 http://dx.doi.org/10.3892/ijo.2011.1304 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	HOSSAIN, MOHAMMAD AKBAR KIM, DONG HWAN JANG, JUNG YOON KANG, YONG JUNG YOON, JEONG-HYUN MOON, JEON-OK CHUNG, HAE YOUNG KIM, GI-YOUNG CHOI, YUNG HYUN COPPLE, BRYAN L. KIM, NAM DEUK
spellingShingle	HOSSAIN, MOHAMMAD AKBAR KIM, DONG HWAN JANG, JUNG YOON KANG, YONG JUNG YOON, JEONG-HYUN MOON, JEON-OK CHUNG, HAE YOUNG KIM, GI-YOUNG CHOI, YUNG HYUN COPPLE, BRYAN L. KIM, NAM DEUK Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
author_facet	HOSSAIN, MOHAMMAD AKBAR KIM, DONG HWAN JANG, JUNG YOON KANG, YONG JUNG YOON, JEONG-HYUN MOON, JEON-OK CHUNG, HAE YOUNG KIM, GI-YOUNG CHOI, YUNG HYUN COPPLE, BRYAN L. KIM, NAM DEUK
author_sort	HOSSAIN, MOHAMMAD AKBAR
title	Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
title_short	Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
title_full	Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
title_fullStr	Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
title_full_unstemmed	Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
title_sort	aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model
description	Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to induce apoptosis in a variety of cancer cells, including colon, prostate, breast and leukemia. Among them, aspirin, a classical NSAID, shows promise in cancer therapy in certain types of cancers. We hypothesized that aspirin might affect the growth of liver cancer cells since liver is the principal site for aspirin metabolism. Therefore, we investigated the effects of aspirin on the HepG2 human hepatocellular carcinoma cell line in vitro and the HepG2 cell xenograft model in BALB/c nude mice. We found that treatment with aspirin inhibited cell growth and induced apoptosis involving both extrinsic and intrinsic pathways as measured by DNA ladder formation, alteration in the Bax/Bcl-2 ratio, activation of the caspase activities and related protein expressions. In vivo antitumor activity assay also showed that aspirin resulted in significant tumor growth inhibition compared to the control. Oral administration of aspirin (100 mg/kg/day) caused a significant reduction in the growth of HepG2 tumors in nude mice. These findings suggest that aspirin may be used as a promising anticancer agent against liver cancer.
publisher	D.A. Spandidos
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584583/
_version_	1611958311473643520

Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model

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