Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa)
In Dictyostelium discoideum (D. discoideum), compounds generating nitric oxide (NO) inhibit its aggregation and differentiation without altering cyclic guanosine monophosphate (cGMP) production. They do it by preventing initiation of cyclic adenosine monophosphate (cAMP) pulses. Furthermore, these c...
| Main Authors: | , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
PAGEPress Publications
2012
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567766/ |
| id |
pubmed-3567766 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-35677662013-02-11 Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) Amaroli, A. Chessa, M.G. Original Paper In Dictyostelium discoideum (D. discoideum), compounds generating nitric oxide (NO) inhibit its aggregation and differentiation without altering cyclic guanosine monophosphate (cGMP) production. They do it by preventing initiation of cyclic adenosine monophosphate (cAMP) pulses. Furthermore, these compounds stimulate adenosine diphosphate (ADP)-ribosylation of a 41 kDa cytosolic protein and regulate the glyceraldehyde-3-phospate dehydrogenase activity. Yet, although D. discoideum cells produce NO at a relatively constant rate at the onset of their developmental cycle, there is still no evidence of the presence of nitric oxide synthase (NOS) enzymes. In this work, we detect the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity in D. discoideum and we characterise it by specific inhibitors and physical-chemical conditions that allegedly distinguish between NOS-related and -unrelated NADPH-d activity. PAGEPress Publications 2012-10-29 /pmc/articles/PMC3567766/ /pubmed/23361243 http://dx.doi.org/10.4081/ejh.2012.e47 Text en ©Copyright A. Amaroli and M.G. Chessa, 2012 This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0). Licensee PAGEPress, Italy |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Amaroli, A. Chessa, M.G. |
| spellingShingle |
Amaroli, A. Chessa, M.G. Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) |
| author_facet |
Amaroli, A. Chessa, M.G. |
| author_sort |
Amaroli, A. |
| title |
Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) |
| title_short |
Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) |
| title_full |
Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) |
| title_fullStr |
Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) |
| title_full_unstemmed |
Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa) |
| title_sort |
detection and characterisation of nad(p)h-diaphorase activity in dictyostelium discoideum cells (protozoa) |
| description |
In Dictyostelium discoideum (D. discoideum), compounds generating nitric oxide (NO) inhibit its aggregation and differentiation without altering cyclic guanosine monophosphate (cGMP) production. They do it by preventing initiation of cyclic adenosine monophosphate (cAMP) pulses. Furthermore, these compounds stimulate adenosine diphosphate (ADP)-ribosylation of a 41 kDa cytosolic protein and regulate the glyceraldehyde-3-phospate dehydrogenase activity. Yet, although D. discoideum cells produce NO at a relatively constant rate at the onset of their developmental cycle, there is still no evidence of the presence of nitric oxide synthase (NOS) enzymes. In this work, we detect the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity in D. discoideum and we characterise it by specific inhibitors and physical-chemical conditions that allegedly distinguish between NOS-related and -unrelated NADPH-d activity. |
| publisher |
PAGEPress Publications |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567766/ |
| _version_ |
1611953511843495936 |