Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF

Perhaps one of the most significant achievements in modern science is the discovery of human induced pluripotent stem cells (hiPSCs), which have paved the way for regeneration therapy using patients’ own cells. Cardiomyocytes differentiated from hiPSCs (hiPSC-CMs) could be used for modelling patient...

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Main Authors:	Ye, Lei, Zhang, Sophia, Greder, Lucas, Dutton, James, Keirstead, Susan A., Lepley, Mike, Zhang, Liying, Kaufman, Dan, Zhang, Jianyi
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542360/

id	pubmed-3542360
recordtype	oai_dc
spelling	pubmed-35423602013-01-16 Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF Ye, Lei Zhang, Sophia Greder, Lucas Dutton, James Keirstead, Susan A. Lepley, Mike Zhang, Liying Kaufman, Dan Zhang, Jianyi Research Article Perhaps one of the most significant achievements in modern science is the discovery of human induced pluripotent stem cells (hiPSCs), which have paved the way for regeneration therapy using patients’ own cells. Cardiomyocytes differentiated from hiPSCs (hiPSC-CMs) could be used for modelling patients with heart failure, for testing new drugs, and for cellular therapy in the future. However, the present cardiomyocyte differentiation protocols exhibit variable differentiation efficiency across different hiPSC lines, which inhibit the application of this technology significantly. Here, we demonstrate a novel myocyte differentiation protocol that can yield a significant, high percentage of cardiac myocyte differentiation (>85%) in 2 hiPSC lines, which makes the fabrication of a human cardiac muscle patch possible. The established hiPSCs cell lines being examined include the transgene integrated UCBiPS7 derived from cord blood cells and non-integrated PCBC16iPS from skin fibroblasts. The results indicate that hiPSC-CMs derived from established hiPSC lines respond to adrenergic or acetylcholine stimulation and beat regularly for greater than 60 days. This data also demonstrates that this novel differentiation protocol can efficiently generate hiPSC-CMs from iPSC lines that are derived not only from fibroblasts, but also from blood mononuclear cells. Public Library of Science 2013-01-10 /pmc/articles/PMC3542360/ /pubmed/23326500 http://dx.doi.org/10.1371/journal.pone.0053764 Text en © 2013 Ye et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ye, Lei Zhang, Sophia Greder, Lucas Dutton, James Keirstead, Susan A. Lepley, Mike Zhang, Liying Kaufman, Dan Zhang, Jianyi
spellingShingle	Ye, Lei Zhang, Sophia Greder, Lucas Dutton, James Keirstead, Susan A. Lepley, Mike Zhang, Liying Kaufman, Dan Zhang, Jianyi Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
author_facet	Ye, Lei Zhang, Sophia Greder, Lucas Dutton, James Keirstead, Susan A. Lepley, Mike Zhang, Liying Kaufman, Dan Zhang, Jianyi
author_sort	Ye, Lei
title	Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
title_short	Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
title_full	Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
title_fullStr	Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
title_full_unstemmed	Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
title_sort	effective cardiac myocyte differentiation of human induced pluripotent stem cells requires vegf
description	Perhaps one of the most significant achievements in modern science is the discovery of human induced pluripotent stem cells (hiPSCs), which have paved the way for regeneration therapy using patients’ own cells. Cardiomyocytes differentiated from hiPSCs (hiPSC-CMs) could be used for modelling patients with heart failure, for testing new drugs, and for cellular therapy in the future. However, the present cardiomyocyte differentiation protocols exhibit variable differentiation efficiency across different hiPSC lines, which inhibit the application of this technology significantly. Here, we demonstrate a novel myocyte differentiation protocol that can yield a significant, high percentage of cardiac myocyte differentiation (>85%) in 2 hiPSC lines, which makes the fabrication of a human cardiac muscle patch possible. The established hiPSCs cell lines being examined include the transgene integrated UCBiPS7 derived from cord blood cells and non-integrated PCBC16iPS from skin fibroblasts. The results indicate that hiPSC-CMs derived from established hiPSC lines respond to adrenergic or acetylcholine stimulation and beat regularly for greater than 60 days. This data also demonstrates that this novel differentiation protocol can efficiently generate hiPSC-CMs from iPSC lines that are derived not only from fibroblasts, but also from blood mononuclear cells.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542360/
_version_	1611946130893963264

Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF

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