Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development
Meso-diencephalic dopaminergic (mdDA) neurons are critical for motor control and cognitive functioning and their loss or dysfunction is associated with disorders such as Parkinson's disease (PD), schizophrenia and addiction. However, relatively little is known about the molecular mechanisms und...
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The Company of Biologists
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507229/ |
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pubmed-35072292012-12-04 Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development Chakrabarty, Koushik Von Oerthel, Lars Hellemons, Anita Clotman, Frédéric Espana, Agnès Groot Koerkamp, Marian Holstege, Frank C. P. Pasterkamp, R. Jeroen Smidt, Marten P. Research Article Meso-diencephalic dopaminergic (mdDA) neurons are critical for motor control and cognitive functioning and their loss or dysfunction is associated with disorders such as Parkinson's disease (PD), schizophrenia and addiction. However, relatively little is known about the molecular mechanisms underlying mdDA neuron development and maintenance. Here, we determined the spatiotemporal map of genes involved in the development of mdDA neurons to gain further insight into their molecular programming. Genome-wide gene expression profiles of the developing ventral mesencephalon (VM) were compared at different developmental stages leading to the identification of novel regulatory roles of neuronal signaling through nicotinic acthylcholine receptors (Chrna6 and Chrnb3 subunits) and the identification of novel transcription factors (Oc2 and 3) involved in the generation of the mdDA neuronal field. We show here that Pitx3, in cooperation with Nurr1, is the critical component in the activation of the Chrna6 and Chrnb3 subunits in mdDA neurons. Furthermore, we provide evidence of two divergent regulatory pathways resulting in the expression of Chrna6 and Chrnb3 respectively. The Company of Biologists 2012-05-17 /pmc/articles/PMC3507229/ /pubmed/23213462 http://dx.doi.org/10.1242/bio.20121230 Text en © 2012. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Chakrabarty, Koushik Von Oerthel, Lars Hellemons, Anita Clotman, Frédéric Espana, Agnès Groot Koerkamp, Marian Holstege, Frank C. P. Pasterkamp, R. Jeroen Smidt, Marten P. |
| spellingShingle |
Chakrabarty, Koushik Von Oerthel, Lars Hellemons, Anita Clotman, Frédéric Espana, Agnès Groot Koerkamp, Marian Holstege, Frank C. P. Pasterkamp, R. Jeroen Smidt, Marten P. Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| author_facet |
Chakrabarty, Koushik Von Oerthel, Lars Hellemons, Anita Clotman, Frédéric Espana, Agnès Groot Koerkamp, Marian Holstege, Frank C. P. Pasterkamp, R. Jeroen Smidt, Marten P. |
| author_sort |
Chakrabarty, Koushik |
| title |
Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| title_short |
Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| title_full |
Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| title_fullStr |
Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| title_full_unstemmed |
Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| title_sort |
genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development |
| description |
Meso-diencephalic dopaminergic (mdDA) neurons are critical for motor control and cognitive functioning and their loss or dysfunction is associated with disorders such as Parkinson's disease (PD), schizophrenia and addiction. However, relatively little is known about the molecular mechanisms underlying mdDA neuron development and maintenance. Here, we determined the spatiotemporal map of genes involved in the development of mdDA neurons to gain further insight into their molecular programming. Genome-wide gene expression profiles of the developing ventral mesencephalon (VM) were compared at different developmental stages leading to the identification of novel regulatory roles of neuronal signaling through nicotinic acthylcholine receptors (Chrna6 and Chrnb3 subunits) and the identification of novel transcription factors (Oc2 and 3) involved in the generation of the mdDA neuronal field. We show here that Pitx3, in cooperation with Nurr1, is the critical component in the activation of the Chrna6 and Chrnb3 subunits in mdDA neurons. Furthermore, we provide evidence of two divergent regulatory pathways resulting in the expression of Chrna6 and Chrnb3 respectively. |
| publisher |
The Company of Biologists |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507229/ |
| _version_ |
1611935680751992832 |