Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice

We present a novel application of optical microangiography (OMAG) imaging technique for visualization of depth-resolved vascular network within retina and choroid as well as measurement of total retinal blood flow in mice. A fast speed spectral domain OCT imaging system at 820nm with a line scan rat...

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Main Authors:	Zhi, Zhongwei, Yin, Xin, Dziennis, Suzan, Wietecha, Tomasz, Hudkins, Kelly L., Alpers, Charles E., Wang, Ruikang K
Format:	Online
Language:	English
Published:	Optical Society of America 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493231/

id	pubmed-3493231
recordtype	oai_dc
spelling	pubmed-34932312012-11-16 Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice Zhi, Zhongwei Yin, Xin Dziennis, Suzan Wietecha, Tomasz Hudkins, Kelly L. Alpers, Charles E. Wang, Ruikang K Optical Coherence Tomography We present a novel application of optical microangiography (OMAG) imaging technique for visualization of depth-resolved vascular network within retina and choroid as well as measurement of total retinal blood flow in mice. A fast speed spectral domain OCT imaging system at 820nm with a line scan rate of 140 kHz was developed to image the posterior segment of eyes in mice. By applying an OMAG algorithm to extract the moving blood flow signals out of the background tissue, we are able to provide true capillary level imaging of the retinal and choroidal vasculature. The microvascular patterns within different retinal layers are presented. An en face Doppler OCT approach [Srinivasan et al., Opt Express 18, 2477 (2010)] was adopted for retinal blood flow measurement. The flow is calculated by integrating the axial blood flow velocity over the vessel area measured in an en face plane without knowing the blood vessel angle. Total retinal blood flow can be measured from both retinal arteries and veins. The results indicate that OMAG has the potential for qualitative and quantitative evaluation of the microcirculation in posterior eye compartments in mouse models of retinopathy and neovascularization. Optical Society of America 2012-10-24 /pmc/articles/PMC3493231/ /pubmed/23162733 http://dx.doi.org/10.1364/BOE.3.002976 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhi, Zhongwei Yin, Xin Dziennis, Suzan Wietecha, Tomasz Hudkins, Kelly L. Alpers, Charles E. Wang, Ruikang K
spellingShingle	Zhi, Zhongwei Yin, Xin Dziennis, Suzan Wietecha, Tomasz Hudkins, Kelly L. Alpers, Charles E. Wang, Ruikang K Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
author_facet	Zhi, Zhongwei Yin, Xin Dziennis, Suzan Wietecha, Tomasz Hudkins, Kelly L. Alpers, Charles E. Wang, Ruikang K
author_sort	Zhi, Zhongwei
title	Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
title_short	Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
title_full	Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
title_fullStr	Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
title_full_unstemmed	Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
title_sort	optical microangiography of retina and choroid and measurement of total retinal blood flow in mice
description	We present a novel application of optical microangiography (OMAG) imaging technique for visualization of depth-resolved vascular network within retina and choroid as well as measurement of total retinal blood flow in mice. A fast speed spectral domain OCT imaging system at 820nm with a line scan rate of 140 kHz was developed to image the posterior segment of eyes in mice. By applying an OMAG algorithm to extract the moving blood flow signals out of the background tissue, we are able to provide true capillary level imaging of the retinal and choroidal vasculature. The microvascular patterns within different retinal layers are presented. An en face Doppler OCT approach [Srinivasan et al., Opt Express 18, 2477 (2010)] was adopted for retinal blood flow measurement. The flow is calculated by integrating the axial blood flow velocity over the vessel area measured in an en face plane without knowing the blood vessel angle. Total retinal blood flow can be measured from both retinal arteries and veins. The results indicate that OMAG has the potential for qualitative and quantitative evaluation of the microcirculation in posterior eye compartments in mouse models of retinopathy and neovascularization.
publisher	Optical Society of America
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493231/
_version_	1611922705357996032

Optical microangiography of retina and choroid and measurement of total retinal blood flow in mice

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