| Summary: | We present a novel application of optical microangiography (OMAG) imaging technique for
visualization of depth-resolved vascular network within retina and choroid as well as measurement of
total retinal blood flow in mice. A fast speed spectral domain OCT imaging system at 820nm with a
line scan rate of 140 kHz was developed to image the posterior segment of eyes in mice. By applying
an OMAG algorithm to extract the moving blood flow signals out of the background tissue, we are able
to provide true capillary level imaging of the retinal and choroidal vasculature. The microvascular
patterns within different retinal layers are presented. An en face Doppler OCT
approach [Srinivasan et al., Opt Express 18, 2477 (2010)] was adopted for retinal blood
flow measurement. The flow is calculated by integrating the axial blood flow velocity over the
vessel area measured in an en face plane without knowing the blood vessel angle.
Total retinal blood flow can be measured from both retinal arteries and veins. The results indicate
that OMAG has the potential for qualitative and quantitative evaluation of the microcirculation in
posterior eye compartments in mouse models of retinopathy and neovascularization.
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