Validation of Reference Genes for Quantitative Expression Analysis by Real-Time RT-PCR in Four Lepidopteran Insects
Quantitative real-time polymerase chain reaction (qPCR) is an efficient and widely used technique to monitor gene expression. Housekeeping genes (HKGs) are often empirically selected as the reference genes for data normalization. However, the suitability of HKGs used as the reference genes has been...
Main Authors: | , , , , |
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Format: | Online |
Language: | English |
Published: |
University of Wisconsin Library
2012
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3481461/ |