Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla

Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing gene expression because of its accuracy, sensitivity, and high throughput. However, the unsuitable choice of reference gene(s) can lead to a misinterpretation of results. We evaluated the stability of 10 candidat...

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Bibliographic Details
Main Authors: He, Yihan, Yan, Hailing, Hua, Wenping, Huang, Yaya, Wang, Zhezhi
Format: Online
Language:English
Published: Frontiers Media S.A. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925707/