Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes
Mast cells (MCs) are heterogeneous cells whose phenotype is modulated by signals received from the local microenvironment. Recent studies have identified the mesenchymal-derived cytokine IL-33 as a potent direct activator of MCs, as well as regulator of their effector phenotype, and have implicated...
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Public Library of Science
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469528/ |
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pubmed-34695282012-10-15 Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes Kaieda, Shinjiro Wang, Jun-Xia Shnayder, Ruslan Fishgal, Nadia Hei, Hillary Lee, Richard T. Stevens, Richard L. Nigrovic, Peter A. Research Article Mast cells (MCs) are heterogeneous cells whose phenotype is modulated by signals received from the local microenvironment. Recent studies have identified the mesenchymal-derived cytokine IL-33 as a potent direct activator of MCs, as well as regulator of their effector phenotype, and have implicated this activity in the ability of mast cells to contribute to murine experimental arthritis. We explored the hypothesis that IL-33 enables participation of synovial MCs in murine K/BxN arthritis by promoting their activation by IgG immune complexes. Compared to wild-type (WT) control mice, transgenic animals lacking the IL-33 receptor ST2 exhibited impaired MC-dependent immune complex-induced vascular permeability (flare) and attenuated K/BxN arthritis. Whereas participation of MCs in this model is mediated by the activating IgG receptor FcγRIII, we pre-incubated bone marrow-derived MCs with IL-33 and found not only direct induction of cytokine release but also a marked increase in FcγRIII-driven production of critical arthritogenic mediators including IL-1β and CXCL2. This “priming” effect was associated with mRNA accumulation rather than altered expression of Fcγ receptors, could be mimicked by co-culture of WT but not ST2−/− MCs with synovial fibroblasts, and was blocked by antibodies against IL-33. In turn, WT but not ST2−/− MCs augmented fibroblast expression of IL-33, forming a positive feedback circuit. Together, these findings confirm a novel role for IL-33 as an amplifier of IgG immune complex-mediated inflammation and identify a potential MC-fibroblast amplification loop dependent on IL-33 and ST2. Public Library of Science 2012-10-11 /pmc/articles/PMC3469528/ /pubmed/23071771 http://dx.doi.org/10.1371/journal.pone.0047252 Text en © 2012 Kaieda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Kaieda, Shinjiro Wang, Jun-Xia Shnayder, Ruslan Fishgal, Nadia Hei, Hillary Lee, Richard T. Stevens, Richard L. Nigrovic, Peter A. |
| spellingShingle |
Kaieda, Shinjiro Wang, Jun-Xia Shnayder, Ruslan Fishgal, Nadia Hei, Hillary Lee, Richard T. Stevens, Richard L. Nigrovic, Peter A. Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes |
| author_facet |
Kaieda, Shinjiro Wang, Jun-Xia Shnayder, Ruslan Fishgal, Nadia Hei, Hillary Lee, Richard T. Stevens, Richard L. Nigrovic, Peter A. |
| author_sort |
Kaieda, Shinjiro |
| title |
Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes |
| title_short |
Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes |
| title_full |
Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes |
| title_fullStr |
Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes |
| title_full_unstemmed |
Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes |
| title_sort |
interleukin-33 primes mast cells for activation by igg immune complexes |
| description |
Mast cells (MCs) are heterogeneous cells whose phenotype is modulated by signals received from the local microenvironment. Recent studies have identified the mesenchymal-derived cytokine IL-33 as a potent direct activator of MCs, as well as regulator of their effector phenotype, and have implicated this activity in the ability of mast cells to contribute to murine experimental arthritis. We explored the hypothesis that IL-33 enables participation of synovial MCs in murine K/BxN arthritis by promoting their activation by IgG immune complexes. Compared to wild-type (WT) control mice, transgenic animals lacking the IL-33 receptor ST2 exhibited impaired MC-dependent immune complex-induced vascular permeability (flare) and attenuated K/BxN arthritis. Whereas participation of MCs in this model is mediated by the activating IgG receptor FcγRIII, we pre-incubated bone marrow-derived MCs with IL-33 and found not only direct induction of cytokine release but also a marked increase in FcγRIII-driven production of critical arthritogenic mediators including IL-1β and CXCL2. This “priming” effect was associated with mRNA accumulation rather than altered expression of Fcγ receptors, could be mimicked by co-culture of WT but not ST2−/− MCs with synovial fibroblasts, and was blocked by antibodies against IL-33. In turn, WT but not ST2−/− MCs augmented fibroblast expression of IL-33, forming a positive feedback circuit. Together, these findings confirm a novel role for IL-33 as an amplifier of IgG immune complex-mediated inflammation and identify a potential MC-fibroblast amplification loop dependent on IL-33 and ST2. |
| publisher |
Public Library of Science |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469528/ |
| _version_ |
1611915533487177728 |