Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus

Most HIV transmission in women occurs through the cervicovaginal mucosa, which is coated by a bacterial biofilm including Lactobacillus. This commensal bacterium plays a role in maintaining healthy mucosa and can be genetically engineered to produce anti-viral peptides. Here, we report a 63% reducti...

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Main Authors:	Lagenaur, Laurel A, Sanders-Beer, Brigitte E, Brichacek, Beda, Pal, Ranajit, Liu, Xiaowen, Liu, Yang, Yu, Rosa, Venzon, David, Lee, Peter P, Hamer, Dean H
Format:	Online
Language:	English
Published:	2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433722/

id	pubmed-3433722
recordtype	oai_dc
spelling	pubmed-34337222012-09-05 Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus Lagenaur, Laurel A Sanders-Beer, Brigitte E Brichacek, Beda Pal, Ranajit Liu, Xiaowen Liu, Yang Yu, Rosa Venzon, David Lee, Peter P Hamer, Dean H Article Most HIV transmission in women occurs through the cervicovaginal mucosa, which is coated by a bacterial biofilm including Lactobacillus. This commensal bacterium plays a role in maintaining healthy mucosa and can be genetically engineered to produce anti-viral peptides. Here, we report a 63% reduction in transmission of a chimeric simian/human immunodeficiency virus (SHIVSF162P3) after repeated vaginal challenges of macaques treated with Lactobacillus jensenii expressing the HIV-1 entry inhibitor cyanovirin-N. Furthermore, peak viral loads in colonized macaques with breakthrough infection were reduced 6-fold. Colonization and prolonged anti-viral protein secretion by the genetically engineered lactobacilli did not cause any increase in proinflammatory markers. These findings lay the foundation for an accessible and durable approach to reduce heterosexual transmission of HIV in women that is coitally independent, inexpensive, and enhances the natural protective effects of the vaginal microflora. 2011-07-06 2011-11 /pmc/articles/PMC3433722/ /pubmed/21734653 http://dx.doi.org/10.1038/mi.2011.30 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lagenaur, Laurel A Sanders-Beer, Brigitte E Brichacek, Beda Pal, Ranajit Liu, Xiaowen Liu, Yang Yu, Rosa Venzon, David Lee, Peter P Hamer, Dean H
spellingShingle	Lagenaur, Laurel A Sanders-Beer, Brigitte E Brichacek, Beda Pal, Ranajit Liu, Xiaowen Liu, Yang Yu, Rosa Venzon, David Lee, Peter P Hamer, Dean H Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus
author_facet	Lagenaur, Laurel A Sanders-Beer, Brigitte E Brichacek, Beda Pal, Ranajit Liu, Xiaowen Liu, Yang Yu, Rosa Venzon, David Lee, Peter P Hamer, Dean H
author_sort	Lagenaur, Laurel A
title	Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus
title_short	Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus
title_full	Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus
title_fullStr	Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus
title_full_unstemmed	Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus
title_sort	prevention of vaginal shiv transmission in macaques by a live recombinant lactobacillus
description	Most HIV transmission in women occurs through the cervicovaginal mucosa, which is coated by a bacterial biofilm including Lactobacillus. This commensal bacterium plays a role in maintaining healthy mucosa and can be genetically engineered to produce anti-viral peptides. Here, we report a 63% reduction in transmission of a chimeric simian/human immunodeficiency virus (SHIVSF162P3) after repeated vaginal challenges of macaques treated with Lactobacillus jensenii expressing the HIV-1 entry inhibitor cyanovirin-N. Furthermore, peak viral loads in colonized macaques with breakthrough infection were reduced 6-fold. Colonization and prolonged anti-viral protein secretion by the genetically engineered lactobacilli did not cause any increase in proinflammatory markers. These findings lay the foundation for an accessible and durable approach to reduce heterosexual transmission of HIV in women that is coitally independent, inexpensive, and enhances the natural protective effects of the vaginal microflora.
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433722/
_version_	1611554176991494144

Prevention of vaginal SHIV transmission in macaques by a live recombinant Lactobacillus

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