Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS

Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS

We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000–4,000 peptide-like features. Out of these, 730 p...

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Main Authors: Hölttä, Mikko, Zetterberg, Henrik, Mirgorodskaya, Ekaterina, Mattsson, Niklas, Blennow, Kaj, Gobom, Johan
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412831/
id pubmed-3412831
recordtype oai_dc
spelling pubmed-34128312012-08-09 Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS Hölttä, Mikko Zetterberg, Henrik Mirgorodskaya, Ekaterina Mattsson, Niklas Blennow, Kaj Gobom, Johan Research Article We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000–4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders. Public Library of Science 2012-08-06 /pmc/articles/PMC3412831/ /pubmed/22880031 http://dx.doi.org/10.1371/journal.pone.0042555 Text en © 2012 Hölttä et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Hölttä, Mikko
Zetterberg, Henrik
Mirgorodskaya, Ekaterina
Mattsson, Niklas
Blennow, Kaj
Gobom, Johan
spellingShingle Hölttä, Mikko
Zetterberg, Henrik
Mirgorodskaya, Ekaterina
Mattsson, Niklas
Blennow, Kaj
Gobom, Johan
Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
author_facet Hölttä, Mikko
Zetterberg, Henrik
Mirgorodskaya, Ekaterina
Mattsson, Niklas
Blennow, Kaj
Gobom, Johan
author_sort Hölttä, Mikko
title Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_short Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_full Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_fullStr Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_full_unstemmed Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_sort peptidome analysis of cerebrospinal fluid by lc-maldi ms
description We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000–4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.
publisher Public Library of Science
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412831/
_version_ 1611547894099214336

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