Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals

Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-γ Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3–21 months. 62% of RD1...

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Main Authors:	Mitchell, Jessica E., Chetty, Shivan, Govender, Pamla, Pillay, Mona, Jaggernath, Manjeetha, Kasmar, Anne, Ndung’u, Thumbi, Klenerman, Paul, Walker, Bruce D., Kasprowicz, Victoria O.
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369919/

id	pubmed-3369919
recordtype	oai_dc
spelling	pubmed-33699192012-06-08 Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals Mitchell, Jessica E. Chetty, Shivan Govender, Pamla Pillay, Mona Jaggernath, Manjeetha Kasmar, Anne Ndung’u, Thumbi Klenerman, Paul Walker, Bruce D. Kasprowicz, Victoria O. Research Article Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-γ Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3–21 months. 62% of RD1-Elispot negative individuals were positive by cultured Elispot. Fluctuations in T cell response were observed with rates of change ranging from −150 to +153 spot-forming cells (SFC)/200,000 PBMC in a 3-month period. To validate these responses we used an RD1-specific real time quantitative PCR assay for monokine-induced by IFN-γ (MIG) and IFN-γ inducible protein-10 (IP10) (MIG: r = 0.6527, p = 0.0114; IP-10: r = 0.6967, p = 0.0056; IP-10+MIG: r = 0.7055, p = 0.0048). During follow-up 30 individuals were placed on ARVs and 4 progressed to active TB. Fluctuations in SFC did not correlate with CD4 count, viral load, treatment initiation, or progression to active TB. The RD1-Elispot appears to have limited value in this setting. Public Library of Science 2012-06-07 /pmc/articles/PMC3369919/ /pubmed/22685549 http://dx.doi.org/10.1371/journal.pone.0037920 Text en Mitchell et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mitchell, Jessica E. Chetty, Shivan Govender, Pamla Pillay, Mona Jaggernath, Manjeetha Kasmar, Anne Ndung’u, Thumbi Klenerman, Paul Walker, Bruce D. Kasprowicz, Victoria O.
spellingShingle	Mitchell, Jessica E. Chetty, Shivan Govender, Pamla Pillay, Mona Jaggernath, Manjeetha Kasmar, Anne Ndung’u, Thumbi Klenerman, Paul Walker, Bruce D. Kasprowicz, Victoria O. Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
author_facet	Mitchell, Jessica E. Chetty, Shivan Govender, Pamla Pillay, Mona Jaggernath, Manjeetha Kasmar, Anne Ndung’u, Thumbi Klenerman, Paul Walker, Bruce D. Kasprowicz, Victoria O.
author_sort	Mitchell, Jessica E.
title	Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
title_short	Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
title_full	Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
title_fullStr	Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
title_full_unstemmed	Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals
title_sort	prospective monitoring reveals dynamic levels of t cell immunity to mycobacterium tuberculosis in hiv infected individuals
description	Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 and CFP-10-specific IFN-γ Elispot assay (RD1-Elispot) on 163 HIV-infected individuals living in a TB-endemic setting. An RD1-Elispot was performed every 3 months for a period of 3–21 months. 62% of RD1-Elispot negative individuals were positive by cultured Elispot. Fluctuations in T cell response were observed with rates of change ranging from −150 to +153 spot-forming cells (SFC)/200,000 PBMC in a 3-month period. To validate these responses we used an RD1-specific real time quantitative PCR assay for monokine-induced by IFN-γ (MIG) and IFN-γ inducible protein-10 (IP10) (MIG: r = 0.6527, p = 0.0114; IP-10: r = 0.6967, p = 0.0056; IP-10+MIG: r = 0.7055, p = 0.0048). During follow-up 30 individuals were placed on ARVs and 4 progressed to active TB. Fluctuations in SFC did not correlate with CD4 count, viral load, treatment initiation, or progression to active TB. The RD1-Elispot appears to have limited value in this setting.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369919/
_version_	1611535333339430912

Prospective Monitoring Reveals Dynamic Levels of T Cell Immunity to Mycobacterium Tuberculosis in HIV Infected Individuals

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