A Molecular Assay for Sensitive Detection of Pathogen-Specific T-Cells

A Molecular Assay for Sensitive Detection of Pathogen-Specific T-Cells

Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-γ production using real time quantitative PCR (qPCR) for two reporters - monokine-induced by IFN-γ (MIG) and the IFN-γ inducible protein-10 (IP10). We developed and validated the assay and applied it...

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Bibliographic Details
Main Authors: Kasprowicz, Victoria O., Mitchell, Jessica E., Chetty, Shivan, Govender, Pamla, Huang, Kuan-Hsiang Gary, Fletcher, Helen A., Webster, Daniel P., Brown, Sebastian, Kasmar, Anne, Millington, Kerry, Day, Cheryl L., Mkhwanazi, Nompumelelo, McClurg, Cheryl, Chonco, Fundisiwe, Lalvani, Ajit, Walker, Bruce D., Ndung'u, Thumbi, Klenerman, Paul
Format: Online
Language:English
Published: Public Library of Science 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154901/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154901/

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