The promoter architectural landscape of the Salmonella PhoP regulon

The DNA-binding protein PhoP controls virulence and Mg2+ homeostasis in the Gram-negative pathogen Salmonella enterica serovar Typhimurium. PhoP regulates expression of a large number of genes that differ both in their ancestry and in the biochemical functions and physiological roles of the encoded...

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Main Authors:	Zwir, Igor, Latifi, Tammy, Perez, J Christian, Huang, Henry, Groisman, Eduardo A
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335776/

id	pubmed-3335776
recordtype	oai_dc
spelling	pubmed-33357762012-11-01 The promoter architectural landscape of the Salmonella PhoP regulon Zwir, Igor Latifi, Tammy Perez, J Christian Huang, Henry Groisman, Eduardo A Research Articles The DNA-binding protein PhoP controls virulence and Mg2+ homeostasis in the Gram-negative pathogen Salmonella enterica serovar Typhimurium. PhoP regulates expression of a large number of genes that differ both in their ancestry and in the biochemical functions and physiological roles of the encoded products. This suggests that PhoP-regulated genes are differentially expressed. To understand how a bacterial activator might generate varied gene expression behaviour, we investigated the cis-acting promoter features (i.e. the number of PhoP binding sites, as well as their orientation and location with respect to the sites bound by RNA polymerase and the sequences that constitute the PhoP binding sites) in 23 PhoP-activated promoters. Our results show that natural PhoP-activated promoters utilize only a limited number of combinations of cis-acting features – or promoter architectures. We determine that PhoP activates transcription by different mechanisms, and that ancestral and horizontally acquired PhoP-activated genes have distinct promoter architectures. Blackwell Publishing Ltd 2012-05 2012-03-27 /pmc/articles/PMC3335776/ /pubmed/22435712 http://dx.doi.org/10.1111/j.1365-2958.2012.08036.x Text en © 2012 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zwir, Igor Latifi, Tammy Perez, J Christian Huang, Henry Groisman, Eduardo A
spellingShingle	Zwir, Igor Latifi, Tammy Perez, J Christian Huang, Henry Groisman, Eduardo A The promoter architectural landscape of the Salmonella PhoP regulon
author_facet	Zwir, Igor Latifi, Tammy Perez, J Christian Huang, Henry Groisman, Eduardo A
author_sort	Zwir, Igor
title	The promoter architectural landscape of the Salmonella PhoP regulon
title_short	The promoter architectural landscape of the Salmonella PhoP regulon
title_full	The promoter architectural landscape of the Salmonella PhoP regulon
title_fullStr	The promoter architectural landscape of the Salmonella PhoP regulon
title_full_unstemmed	The promoter architectural landscape of the Salmonella PhoP regulon
title_sort	promoter architectural landscape of the salmonella phop regulon
description	The DNA-binding protein PhoP controls virulence and Mg2+ homeostasis in the Gram-negative pathogen Salmonella enterica serovar Typhimurium. PhoP regulates expression of a large number of genes that differ both in their ancestry and in the biochemical functions and physiological roles of the encoded products. This suggests that PhoP-regulated genes are differentially expressed. To understand how a bacterial activator might generate varied gene expression behaviour, we investigated the cis-acting promoter features (i.e. the number of PhoP binding sites, as well as their orientation and location with respect to the sites bound by RNA polymerase and the sequences that constitute the PhoP binding sites) in 23 PhoP-activated promoters. Our results show that natural PhoP-activated promoters utilize only a limited number of combinations of cis-acting features – or promoter architectures. We determine that PhoP activates transcription by different mechanisms, and that ancestral and horizontally acquired PhoP-activated genes have distinct promoter architectures.
publisher	Blackwell Publishing Ltd
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335776/
_version_	1611524447896862720

The promoter architectural landscape of the Salmonella PhoP regulon

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