Cell Cycle-Dependent Mobility of Cdc45 Determined in vivo by Fluorescence Correlation Spectroscopy

Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our da...

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Bibliographic Details
Main Authors: Broderick, Ronan, Ramadurai, Sivaramakrishnan, Tóth, Katalin, Togashi, Denisio M., Ryder, Alan G., Langowski, Jörg, Nasheuer, Heinz Peter
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334904/

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