BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways

Our preliminary screening has shown that curcumin derivative BDMC33 [2,6-bis(2,5-dimethoxybenzylidene)cyclohexanone] exerted promising nitric oxide inhibitory activity in activated macrophages. However, the molecular basis and mechanism for its pharmacological action is yet to be elucidated. The aim...

Full description

Bibliographic Details
Main Authors:	Lee, Ka-Heng, Chow, Yuh-Lit, Sharmili, Vidyadaran, Abas, Faridah, Alitheen, Noorjahan Banu Mohamed, Shaari, Khozirah, Israf, Daud Ahmad, Lajis, Nordin Haji, Syahida, Ahmad
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317699/

id	pubmed-3317699
recordtype	oai_dc
spelling	pubmed-33176992012-04-09 BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways Lee, Ka-Heng Chow, Yuh-Lit Sharmili, Vidyadaran Abas, Faridah Alitheen, Noorjahan Banu Mohamed Shaari, Khozirah Israf, Daud Ahmad Lajis, Nordin Haji Syahida, Ahmad Article Our preliminary screening has shown that curcumin derivative BDMC33 [2,6-bis(2,5-dimethoxybenzylidene)cyclohexanone] exerted promising nitric oxide inhibitory activity in activated macrophages. However, the molecular basis and mechanism for its pharmacological action is yet to be elucidated. The aim of this study was to investigate the anti-inflammatory properties of BDMC33 and elucidate its underlying mechanism action in macrophage cells. Our current study demonstrated that BDMC33 inhibits the secretion of major pro-inflammatory mediators in stimulated macrophages, and includes NO, TNF-α and IL-1β through interference in both nuclear factor kappaB (NF-κB) and mitogen activator protein kinase (MAPK) signaling cascade in IFN-γ/LPS-stimulated macrophages. Moreover, BDMC33 also interrupted LPS signaling through inhibiting the surface expression of CD-14 accessory molecules. In addition, the inhibitory action of BDMC33 not only restricted the macrophages cell (RAW264.7), but also inhibited the secretion of NO and TNF-α in IFN-γ/LPS-challenged microglial cells (BV-2). The experimental data suggests the inflammatory action of BDMC33 on activated macrophage-like cellular systems, which could be used as a future therapeutic agent in the management of chronic inflammatory diseases. Molecular Diversity Preservation International (MDPI) 2012-03-06 /pmc/articles/PMC3317699/ /pubmed/22489138 http://dx.doi.org/10.3390/ijms13032985 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lee, Ka-Heng Chow, Yuh-Lit Sharmili, Vidyadaran Abas, Faridah Alitheen, Noorjahan Banu Mohamed Shaari, Khozirah Israf, Daud Ahmad Lajis, Nordin Haji Syahida, Ahmad
spellingShingle	Lee, Ka-Heng Chow, Yuh-Lit Sharmili, Vidyadaran Abas, Faridah Alitheen, Noorjahan Banu Mohamed Shaari, Khozirah Israf, Daud Ahmad Lajis, Nordin Haji Syahida, Ahmad BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways
author_facet	Lee, Ka-Heng Chow, Yuh-Lit Sharmili, Vidyadaran Abas, Faridah Alitheen, Noorjahan Banu Mohamed Shaari, Khozirah Israf, Daud Ahmad Lajis, Nordin Haji Syahida, Ahmad
author_sort	Lee, Ka-Heng
title	BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways
title_short	BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways
title_full	BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways
title_fullStr	BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways
title_full_unstemmed	BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways
title_sort	bdmc33, a curcumin derivative suppresses inflammatory responses in macrophage-like cellular system: role of inhibition in nf-κb and mapk signaling pathways
description	Our preliminary screening has shown that curcumin derivative BDMC33 [2,6-bis(2,5-dimethoxybenzylidene)cyclohexanone] exerted promising nitric oxide inhibitory activity in activated macrophages. However, the molecular basis and mechanism for its pharmacological action is yet to be elucidated. The aim of this study was to investigate the anti-inflammatory properties of BDMC33 and elucidate its underlying mechanism action in macrophage cells. Our current study demonstrated that BDMC33 inhibits the secretion of major pro-inflammatory mediators in stimulated macrophages, and includes NO, TNF-α and IL-1β through interference in both nuclear factor kappaB (NF-κB) and mitogen activator protein kinase (MAPK) signaling cascade in IFN-γ/LPS-stimulated macrophages. Moreover, BDMC33 also interrupted LPS signaling through inhibiting the surface expression of CD-14 accessory molecules. In addition, the inhibitory action of BDMC33 not only restricted the macrophages cell (RAW264.7), but also inhibited the secretion of NO and TNF-α in IFN-γ/LPS-challenged microglial cells (BV-2). The experimental data suggests the inflammatory action of BDMC33 on activated macrophage-like cellular systems, which could be used as a future therapeutic agent in the management of chronic inflammatory diseases.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317699/
_version_	1611518621970857984

BDMC33, A Curcumin Derivative Suppresses Inflammatory Responses in Macrophage-Like Cellular System: Role of Inhibition in NF-κB and MAPK Signaling Pathways

Similar Items