Computational identification of microRNA gene loci and precursor microRNA sequences in CHO cell lines

► We mapped all known mature CHO miRNAs to two CHO-K1 reference genomes. ► 212 unique genomic miRNA loci and the respective precursor miRNA sequences were identified. ► The genomic loci of 4 polycistronic miRNA cluster were confirmed by PCR. ► The identified sequences were analyzed for SNPs and cons...

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Bibliographic Details
Main Authors: Hackl, Matthias, Jadhav, Vaibhav, Jakobi, Tobias, Rupp, Oliver, Brinkrolf, Karina, Goesmann, Alexander, Pühler, Alfred, Noll, Thomas, Borth, Nicole, Grillari, Johannes
Format: Online
Language:English
Published: Elsevier Science Publishers 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314935/