Portrait of Candida albicans Adherence Regulators
Cell-substrate adherence is a fundamental property of microorganisms that enables them to exist in biofilms. Our study focuses on adherence of the fungal pathogen Candida albicans to one substrate, silicone, that is relevant to device-associated infection. We conducted a mutant screen with a quantit...
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| Format: | Online |
| Language: | English |
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Public Library of Science
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280983/ |
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pubmed-3280983 |
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pubmed-32809832012-02-22 Portrait of Candida albicans Adherence Regulators Finkel, Jonathan S. Xu, Wenjie Huang, David Hill, Elizabeth M. Desai, Jigar V. Woolford, Carol A. Nett, Jeniel E. Taff, Heather Norice, Carmelle T. Andes, David R. Lanni, Frederick Mitchell, Aaron P. Research Article Cell-substrate adherence is a fundamental property of microorganisms that enables them to exist in biofilms. Our study focuses on adherence of the fungal pathogen Candida albicans to one substrate, silicone, that is relevant to device-associated infection. We conducted a mutant screen with a quantitative flow-cell assay to identify thirty transcription factors that are required for adherence. We then combined nanoString gene expression profiling with functional analysis to elucidate relationships among these transcription factors, with two major goals: to extend our understanding of transcription factors previously known to govern adherence or biofilm formation, and to gain insight into the many transcription factors we identified that were relatively uncharacterized, particularly in the context of adherence or cell surface biogenesis. With regard to the first goal, we have discovered a role for biofilm regulator Bcr1 in adherence, and found that biofilm regulator Ace2 is a major functional target of chromatin remodeling factor Snf5. In addition, Bcr1 and Ace2 share several target genes, pointing to a new connection between them. With regard to the second goal, our findings reveal existence of a large regulatory network that connects eleven adherence regulators, the zinc-response regulator Zap1, and approximately one quarter of the predicted cell surface protein genes in this organism. This limited yet sensitive glimpse of mutant gene expression changes had thus defined one of the broadest cell surface regulatory networks in C. albicans. Public Library of Science 2012-02-16 /pmc/articles/PMC3280983/ /pubmed/22359502 http://dx.doi.org/10.1371/journal.ppat.1002525 Text en Finkel et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Finkel, Jonathan S. Xu, Wenjie Huang, David Hill, Elizabeth M. Desai, Jigar V. Woolford, Carol A. Nett, Jeniel E. Taff, Heather Norice, Carmelle T. Andes, David R. Lanni, Frederick Mitchell, Aaron P. |
| spellingShingle |
Finkel, Jonathan S. Xu, Wenjie Huang, David Hill, Elizabeth M. Desai, Jigar V. Woolford, Carol A. Nett, Jeniel E. Taff, Heather Norice, Carmelle T. Andes, David R. Lanni, Frederick Mitchell, Aaron P. Portrait of Candida albicans Adherence Regulators |
| author_facet |
Finkel, Jonathan S. Xu, Wenjie Huang, David Hill, Elizabeth M. Desai, Jigar V. Woolford, Carol A. Nett, Jeniel E. Taff, Heather Norice, Carmelle T. Andes, David R. Lanni, Frederick Mitchell, Aaron P. |
| author_sort |
Finkel, Jonathan S. |
| title |
Portrait of Candida albicans Adherence Regulators |
| title_short |
Portrait of Candida albicans Adherence Regulators |
| title_full |
Portrait of Candida albicans Adherence Regulators |
| title_fullStr |
Portrait of Candida albicans Adherence Regulators |
| title_full_unstemmed |
Portrait of Candida albicans Adherence Regulators |
| title_sort |
portrait of candida albicans adherence regulators |
| description |
Cell-substrate adherence is a fundamental property of microorganisms that enables them to exist in biofilms. Our study focuses on adherence of the fungal pathogen Candida albicans to one substrate, silicone, that is relevant to device-associated infection. We conducted a mutant screen with a quantitative flow-cell assay to identify thirty transcription factors that are required for adherence. We then combined nanoString gene expression profiling with functional analysis to elucidate relationships among these transcription factors, with two major goals: to extend our understanding of transcription factors previously known to govern adherence or biofilm formation, and to gain insight into the many transcription factors we identified that were relatively uncharacterized, particularly in the context of adherence or cell surface biogenesis. With regard to the first goal, we have discovered a role for biofilm regulator Bcr1 in adherence, and found that biofilm regulator Ace2 is a major functional target of chromatin remodeling factor Snf5. In addition, Bcr1 and Ace2 share several target genes, pointing to a new connection between them. With regard to the second goal, our findings reveal existence of a large regulatory network that connects eleven adherence regulators, the zinc-response regulator Zap1, and approximately one quarter of the predicted cell surface protein genes in this organism. This limited yet sensitive glimpse of mutant gene expression changes had thus defined one of the broadest cell surface regulatory networks in C. albicans. |
| publisher |
Public Library of Science |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280983/ |
| _version_ |
1611506538693787648 |