Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification
CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneou...
| Main Authors: | , , , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Nature Publishing Group
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3271364/ |