Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition

MicroRNA (miRNA) sponges are transcripts with repeated miRNA antisense sequences that can sequester miRNAs from endogenous targets. MiRNA sponges are valuable tools for miRNA loss-of-function studies both in vitro and in vivo. We developed a fast and flexible method to generate miRNA sponges and tes...

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Bibliographic Details
Main Authors: Kluiver, Joost, Gibcus, Johan H., Hettinga, Chris, Adema, Annelies, Richter, Mareike K. S., Halsema, Nancy, Slezak-Prochazka, Izabella, Ding, Ye, Kroesen, Bart-Jan, van den Berg, Anke
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253070/