Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy
We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded...
| Main Authors: | , , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Optical Society of America
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207382/ |