Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

During the last few years scientists became increasingly aware that average data obtained from microbial population based experiments are not representative of the behavior, status or phenotype of single cells. Due to this new insight the number of single cell studies rises continuously (for recent...

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Bibliographic Details
Main Authors: de Jong, Imke G., Beilharz, Katrin, Kuipers, Oscar P., Veening, Jan- Willem
Format: Online
Language:English
Published: MyJove Corporation 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197447/
Description
Summary:During the last few years scientists became increasingly aware that average data obtained from microbial population based experiments are not representative of the behavior, status or phenotype of single cells. Due to this new insight the number of single cell studies rises continuously (for recent reviews see 1,2,3). However, many of the single cell techniques applied do not allow monitoring the development and behavior of one specific single cell in time (e.g. flow cytometry or standard microscopy).