Single Cell FRET Analysis for the Identification of Optimal FRET-Pairs in Bacillus subtilis Using a Prototype MEM-FLIM System

Protein-protein interactions can be studied in vitro, e.g. with bacterial or yeast two-hybrid systems or surface plasmon resonance. In contrast to in vitro techniques, in vivo studies of protein-protein interactions allow examination of spatial and temporal behavior of such interactions in their nat...

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Bibliographic Details
Main Authors: Detert Oude Weme, Ruud G. J., Kovács, Ákos T., de Jong, Sander J. G., Veening, Jan-Willem, Siebring, Jeroen, Kuipers, Oscar P.
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401445/