Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
An overview of the methods used for high-throughput cloning and protein-expression screening of SSGCID hexahistidine recombinant proteins is provided. It is demonstrated that screening for recombinant proteins that are highly recoverable from immobilized metal-affinity chromatography improves the li...
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| Format: | Online |
| Language: | English |
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International Union of Crystallography
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169392/ |
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pubmed-31693922011-09-21 Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success Choi, Ryan Kelley, Angela Leibly, David Nakazawa Hewitt, Stephen Napuli, Alberto Van Voorhis, Wesley Laboratory Communications An overview of the methods used for high-throughput cloning and protein-expression screening of SSGCID hexahistidine recombinant proteins is provided. It is demonstrated that screening for recombinant proteins that are highly recoverable from immobilized metal-affinity chromatography improves the likelihood that a protein will produce a structure. International Union of Crystallography 2011-08-13 /pmc/articles/PMC3169392/ /pubmed/21904040 http://dx.doi.org/10.1107/S1744309111017374 Text en © Choi et al. 2011 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Choi, Ryan Kelley, Angela Leibly, David Nakazawa Hewitt, Stephen Napuli, Alberto Van Voorhis, Wesley |
| spellingShingle |
Choi, Ryan Kelley, Angela Leibly, David Nakazawa Hewitt, Stephen Napuli, Alberto Van Voorhis, Wesley Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| author_facet |
Choi, Ryan Kelley, Angela Leibly, David Nakazawa Hewitt, Stephen Napuli, Alberto Van Voorhis, Wesley |
| author_sort |
Choi, Ryan |
| title |
Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| title_short |
Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| title_full |
Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| title_fullStr |
Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| title_full_unstemmed |
Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| title_sort |
immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success |
| description |
An overview of the methods used for high-throughput cloning and protein-expression screening of SSGCID hexahistidine recombinant proteins is provided. It is demonstrated that screening for recombinant proteins that are highly recoverable from immobilized metal-affinity chromatography improves the likelihood that a protein will produce a structure. |
| publisher |
International Union of Crystallography |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169392/ |
| _version_ |
1611474428425666560 |