Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography
Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSki...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Brazilian Society of Chemical Engineering
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/1/Recovery%20of%20cyclodextrin%20glucanotransferase.pdf |